Q: Early gene-cloning experiments involved insertion at one restriction site in the vector; for…
A: Answer: Introduction: Restriction endonucleases enzymes which cut DNA at precise recognition sites.…
Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?
A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…
Q: You have sequenced the genome of a new E. coli strain that you discovered during the metagenomics…
A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…
Q: How do you clone a 4 kb promoter from a genomic cell preparation?
A: Genome is the complete set of genetic material present in the nucleus of a cell. It houses all the…
Q: The observation that some genes are mosaics, composed of parts of other genes can be explained by…
A: Mosaicism or we can say genetic mosaicism is a condition in multi-cell life form in which a solitary…
Q: The figure below shows RNA-Seq data (RED) for the D. melanogaster transformer (tra) gene obtained…
A: DNA is made up of nucleotides. DNA consists of exons which are the coding sequences and introns…
Q: What are the use and the advantages of discovering CRISPR Cas-9 of Jennifer Anne Doudna in research…
A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chains running in…
Q: When a chromosomal rearrangement, such as an inversion, places a known gene into or next to a…
A: There are 4 types of chromosomal rearrangements namely - duplication, deletion, inversion,…
Q: Like many biotechnologies, CRISPR/Cas9 was originally discovered as system that naturally occurs in…
A: Biotechnology is the branch of biology which deals with the utilisation of cells and biomolecular…
Q: Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9.…
A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition,…
Q: Examine the “RNA-Seq Coverage” and the “FlyBase Genes” tracks in the Genome Browser from left to…
A: Introduction: RNA is a single-stranded molecule in many of its biological roles and consists of a…
Q: what is CRISPR Cas9 and the process of it in the human body?
A: CRISPR Cas9: A unique genome editing techno tool which enables researchers worldwide to…
Q: How can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted…
A: The generation of new plants with improvised constructs of features that are desirable is based on…
Q: The sequence at one end of one strand of the Drosophilatransposon Mariner is shown below (dots…
A: The genes are the sequence of nucleotides that are present on the chromosomes and encode for a…
Q: Describe one way in which DNA double stranded break repair that occurs because of X-ray damage…
A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…
Q: Under most practical circumstances, Crispr-cas9 technology can edit a eukaryote's genome (to replace…
A: Correct response to this particular question would be, 80%.
Q: Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in…
A: CRISPR-Cas9 is a genome editing tool. It helps researchers to edit or change the gene from the…
Q: List other methods of reverse genetics available in S. cerevisiae, Drosophila, Arabidopsis, and…
A: Saccharomyces cerevisiae commonly known as the yeast are micro organism with application in…
Q: What does CRISPR-Cas stand for and how does it work? What are the molecules involved with CRISPR-Cas
A: Introduction Genome editing:- It is also known as gene editing or genome engineering. It is a type…
Q: How can proteomics identify differences between the numberof protein-coding genes predicted for a…
A: Proteomics is defined as the characterization of proteomes, sets of proteins that are synthesized in…
Q: The Cas9 enzyme requires a short sequence called a PAM (Protospacer Adjacent Motif) for cleaving the…
A: A protospacer adjacent motif (PAM) is a 2–6 base pair DNA sequence tareted by Cas9 nuclease.
Q: Briefly describe the experiment that demonstrates that the transposition of the Ty1 element in yeast…
A: Ty1 is a retrotransposon which is transpose via RNA intermediate, and this type of element found in…
Q: What is CRISPR-Cas9? What is one advantage and one disadvantage of this technology?
A: Gene editing is a technology in which Genetic material of an organism is altered and remodelled.…
Q: What is CRISPR/ Cas 9 and how is CRISPR/ Cas 9 used to repair mutant genes.
A: CRISPR/Cas 9 stands for clustered regularly interspersed short palindromic sequences and CRISPR…
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A: Different species of plants can be crossed together to produce hybrid plants using the process of…
Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?
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Q: Some expression plasmids have small peptide or protein tags in front of and/or after the cloning…
A: An expression plasmid or expression vector is a plasmid which is designed for gene expression in…
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A: The chromosomal theory of inheritance provides the idea that genes are located on the chromosomes.…
Q: what are BIOLOGICAL IMPLICATIONS OF CRISPR-CAS9?
A: CRISPR- CAS9 is used to create animal models to mimic humans especially in case of diseases where…
Q: An integrated gene X inserted into the yeast genome near a telomere. Will this strategy result in…
A: Introduction Telomers are the farthest most ends of the chromosomes which contains the repeated…
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A: Transgenic cows are produced by DNA cloning methods in which a foreign gene is inserted into the…
Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…
A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…
Q: How can the efficiency of homologous recombination be improved when using CRISPR-Cas9, compared to…
A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a technique derived from…
Q: How can CRISPR/cas9 be used to insert anthocyanins into a mango plant
A: Horticultural crops, including fruit, vegetable, and ornamental plants are an important component of…
Q: Identification and quantification of expressed genes initially requires a. reverse transcription of…
A: Gene is a basic unit of heredity and a sequence of nucleotides in DNA that encodes the Synthesis of…
Q: yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what…
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Q: In maize, a Ds or Ac transposon can alter the function of genes at or near the site of transposon…
A: Transposons has the capability to induce various alteratins in the DNA or chromosomes thay may lead…
Q: More than half of the genome of Arabidopsis thaliana consists of duplicated sequences. What…
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Q: How else can it be proven that transformants have the topA-cysB fragment apart from size analysis…
A: A transformant cell is a cell that has received additional genetic material, either experimentally…
Q: elect molecular outcomes that can be achieved with the CRISPR-Cas9 system (Check all that apply.)…
A: CRISPR- Cas9 is a genome-editing tool which is derived from bacteria.
Q: Genome-wide RNAi screens target expression of > 16,000 genes. Explain how each of these 16,000+…
A: The control of gene expression in a cell to inhibit the expression of a specific gene is known as…
Q: After Drosophila DNA has been treated with a restrictionenzyme, the fragments are inserted into…
A: Cloning is a particular technique scientists use to make exact genetic copies of living organisms.…
Q: You want to clone a eukaryotic gene and express the corresponding protein in yeast. However, the…
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Q: #3) Ligase catalyzes a reaction between the 5' phosphate and the 3' hydroxyl groups at the end of…
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Q: What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a…
A: BASIC INFORMATION GENE MAPPING This is a technique to identify the position of elements in the…
Q: How is the sgRNA different from certain components of the bacterial defense system described?
A: Introduction CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. This is…
2) How can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted into a genome?
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- How can CRISPR/cas9 be used to insert anthocyanins into a mango plant5. Consider the following examples that include both haploids and partial diploids and explain in each scenario a. whether the repressor can bind and regulate expression and b. whether or not the lac operon is expressed. For partial diploids the plasmid is indicated by the F’. I + O +Z + Y + / F’ I+ O + Z + Y + I - O + Z + Y + / F’ I+ O + Z + Y + I - O + Z + Y + I + O c Z + Y +Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9. Which approach leads to genetic mosaicism? What types of germline gene editing is approved and what type is banned?
- Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?How can I develop a vector to express a bacterial gene (2,500 bp in size) in E. coli. How do I know if the gene of interest is in the vector? What method can I use to get the vector into E. coli?What is CRISPR/ Cas 9 and how is CRISPR/ Cas 9 used to repair mutant genes.
- Explain how the VIN3/PRC2 complex specifically binds to theFLC gene.What is o A vector derived from the tumor-inducing (Ti) plasmid of the bacterium Agrobacterium tumefaciens is the basis for an efficient method for introducing transgenes into plants?It has been shown that infectious agents such as viruses often exert a dramatic effect on their host cell’s genome architecture. In many cases, viruses induce methylation of host DNA sequences in order to enhance their infectivity. What specific host gene functions would you consider as strong candidates for such methylation by infecting viruses?