The LEAST critical part of a plasmid needed to create a transgenic cow that expresses a foreign protein in its milk is: O A. A milk-specific promoter sequence. O B. A start codon. O C.A polyadenylation signal. O D. An ampicillin-resistance gene. O E. A stop codon.
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- Part A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingA mutagen changes a C-G basepair in the genome to an A-T basepair. This is an example of... A different mutagen changes a C-G basepair in the genome to a T-A basepair. This is an example of... a. transition b. inversion c. transversion d. epigeneticYou are on a research project to study rice. Your want to use reverse transcription PCR to amplify the OsMEI28 cDNA from the plant and create a plasmid that contains the cDNA sequence in a vector. Briefly describe the steps that you would need to take.
- In an EMSA, the binding of a protein to DNAa. prevents the DNA from being digested with a restrictionenzyme.b. causes the DNA to migrate more slowly through a gel.c. causes the DNA to migrate more quickly through a gel.d. inhibits the expression of any genes within the DNA.Cheese-makers use an enzyme, normally found in cow stomachs, to start the conversion of milk to cheese. The cow gene that codes for this enzyme has been inserted into the genetic material of a microbe, Aspergillus niger. Why are Aspergillus niger cells able to correctly transcribe and translate a cow gene and make this enzyme? A DNA is a double helix. B Cow cells must have contaminated and cross-bred with the A. niger cells. C The genetic code is universalIn order to express a eukaryotic gene in bacteria, which components are necessary for an expression vector that are not found in a regular plasmid? Select all that apply. a.)bacterial ribosome-bind sites b.)origin of replication c.)bacterial promoter d.)centromere
- Plants are more readily manipulated by genetic engineeringthan are animals because(A) plant genes do not contain introns.(B) more vectors are available for transferring recombinantDNA into plant cells.(C) a somatic plant cell can often give rise to a completeplant.(D) plant cells have larger nucleiAlthough SINES are nonautonomous, they do occasionally move from one genomic site to another. How is this usually achieved? a. Using another TE's insertion sequence b. Using another TE's restriction enzyme c. Using another TE's transposase d. Using another TE's reverse transcriptaseWhich of the following is a function of a restriction endonuclease? a. It cleaves a DNA molecule at a specific site. b. It cleaves a DNA molecule at random locations. c. It removes specific codons from an mRNA molecule. d. It removes specific exons from an mRNA molecule.
- The experimental insertion of a fragment of frog DNA into a bacterial plasmid produces a(n) ________ molecule; if this fragment contains a gene, the process is most accurately referred to as ________. a.recombinant; gene cloning b. diploid; splicing c. Hybrid; fusion d.Eukaryo-bacterial; genetic engineering e. Mutant; biosynthesiswhich of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase d. a growth medium with carefully selected ingredients that take advantage of selectable markers. e. none of the above.Explain the difference between gene modification and gene addition.Are the following examples of gene modification or gene addition?A. A mouse model to study cystic fibrosisB. Introduction of a pesticide-resistance gene into corn using theT-DNA vector of A. tumefaciens