How can you tell if you have had a successful induced transformation using: "C" = control, "T" =transformation mixture 50ul of CaCl2 competent coli, in "C" and "T" 5 μL of plasmid DNA (pUC 19), just "T" 250 μL of SOC medium, in "C" and "T" 10 μL of “C” onto a TSA + ampicillin plate "C" 100 μL of “C” onto a TSA + ampicillin plate "T" I have included a picture of the two plates.  Thank You

Principles Of Radiographic Imaging: An Art And A Science
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Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
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How can you tell if you have had a successful induced transformation using: "C" = control, "T" =transformation mixture

50ul of CaCl2 competent coli, in "C" and "T"

5 μL of plasmid DNA (pUC 19), just "T"

250 μL of SOC medium, in "C" and "T"

10 μL of “C” onto a TSA + ampicillin plate "C"

100 μL of “C” onto a TSA + ampicillin plate "T"

I have included a picture of the two plates.  Thank You

"C"
¡T"
Transcribed Image Text:"C" ¡T"
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