How can you tell if you have had a successful induced transformation using: "C" = control, "T" =transformation mixture 50ul of CaCl2 competent coli, in "C" and "T" 5 μL of plasmid DNA (pUC 19), just "T" 250 μL of SOC medium, in "C" and "T" 10 μL of “C” onto a TSA + ampicillin plate "C" 100 μL of “C” onto a TSA + ampicillin plate "T" I have included a picture of the two plates. Thank You
How can you tell if you have had a successful induced transformation using: "C" = control, "T" =transformation mixture 50ul of CaCl2 competent coli, in "C" and "T" 5 μL of plasmid DNA (pUC 19), just "T" 250 μL of SOC medium, in "C" and "T" 10 μL of “C” onto a TSA + ampicillin plate "C" 100 μL of “C” onto a TSA + ampicillin plate "T" I have included a picture of the two plates. Thank You
Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter3: Electricity
Section: Chapter Questions
Problem 7RQ
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How can you tell if you have had a successful induced transformation using: "C" = control, "T" =transformation mixture
50ul of CaCl2 competent coli, in "C" and "T"
5 μL of plasmid DNA (pUC 19), just "T"
250 μL of SOC medium, in "C" and "T"
10 μL of “C” onto a TSA + ampicillin plate "C"
100 μL of “C” onto a TSA + ampicillin plate "T"
I have included a picture of the two plates. Thank You
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