How do you prepare 500 mL pH 6 Citric acid/Phosphate buffer in the laboratory. Please explain briefly.

Q: 0.0050 M KOH PH VALUE

A: KOH stands for potassium hydroxide. It is an inorganic compound with the chemical formula of KOH.

Q: How much of a 10x buffer should be added to 900ml of water to make a 1x solution?

A: In this question, we are given with- 1. Concentration of stock solution= 10X 2. Required…

Q: For the following, calculate pka, and pka, of 1 Glycine, determine the 2 buffering regions, and…

A: Glycine is an amino acid with H-atom as the side chain. The isoelectric point of a amino acid (pI)…

Q: A 100-mL buffer solution with pH of 4.80 is prepared as a stock solution. Using this stock buffer…

A: Hi! Thank you for the question. We are authorized to answer two subparts at a time, since you have…

Q: What is the molarity of a 30% solution of NaCI

A: Let us first define the percentage solution : 30% NaCl solution= 30% w/v solution = 30g/100 ml.Since…

Q: If a pharmacist needs to prepare a bacterial suspension with a concentration of 2.4x10^2 cfu using 1…

A: A colony-forming unit is the number of viable or alive cells in cell suspension that have the…

Q: What volume of 10X TBE buffer should you use to make 100mL of 1X TBE buffer? 10mL 1mL 0.1mL 100mL

A: TBE buffer stands for Tris-Borate-EDTA buffer. It is used for both agarose and polyacrylamide gel…

Q: Which of the following represents a Buffer solution?

A: Buffer solution can be defined as the solution which resists changes in pH when acid or alkali is…

Q: What are the pH values for the following solutions? (a) 0.1 MHCl (b) 0.1 M NaOH (c) 0.05 M HCl (d)…

A: pH is the scale used to specify the acidity or basicity of an aqueous solution. It is the negative…

Q: Explain how do you prepare a 25-mL solution of 1 mg/mL cholesterol stock?

A: Given Values: Volume = 25 ml Concentration = 1 mg/ml

Q: How much water must be added to 300 mL of an aqueous solution of 0.2 M acetic acid in order to…

A: Given : 0.2 M acetic acid solution , volume 300 ml Let C1 = 0.2 M C2 = concentration of acetic acid…

Q: Ammonia-containing window cleaners are considered pH-neutral substances true or false?

A: As chemicals are dissolved in water, the pH level of the mixture is either acidic or basic. pH level…

Q: What is the pH of the buffer 0.17 M Na2HPO4/0.25 M KH2PO4?

A: Buffer is the mixture of strong acid and salt of that acid (base). If we add either strong acids or…

Q: Convert 12 pints to an equivalent volume in quarts.

A: In USA there's a dry pint and a liquid pint which measure different.Dry pint measures 550.6 ml and a…

Q: How many grams are in 1 mL for the following solution?   NS _________ this is the whole question

A: NS or Normal saline: It is nothing but a mixture of sodium chloride (NaCl) and water that is…

Q: Why does buffers is significant inside a human body?

A: A buffer system is a solution that resists a change in pH when acids or bases are added to it.…

Q: Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.

A: A buffer is defined as the mixture of a weak acid and its conjugate base or weak base and its…

Q: give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete…

A: Introduction: A serial dilution is any dilution in which the concentration decreases by the same…

Q: What volumes of 50 mM CH3COOH and 30 mM NaCH3COO solutions are needed to prepare 2L of 20 mM acetate…

A: Given Values: The pH of acetate buffer = 3.6 Concentration and volume of acetate buffer = 20 mM and…

Q: How would you make wash buffer with 0.05M Tris containing 0.05M NaCl in 200ml at pH of 8.0

A: A solution is a mixture of one or more than one solute mixed in a solvent. The molarity of the…

Q: What is the functional difference between TAE and TBE buffer?

A: A buffer is a solution capable of withstanding pH changes even when a base or acid solution is…

Q: Make the following prescription isotonic with lacrimal fluid: 0.06 g 0.30 g Ephedrine sulfate (E =…

A: A drug, often known as a medicine, is a chemical compound that has a biological effect on the human…

Q: Define the following: - pH - Buffer - pKa

A: An acid-base reaction takes place between an acid and a base and is used to determine pH. An acid is…

Q: A patient is prescribed 15mmol of potassium chloride injection. The ampoule contains 20mmol in 10ml.…

A: Given data    A 10 ml ampule contains = 20 mmol

Q: What kind of buffer would you make with a optimal activity at ph 4.30?

A: A buffer is defined as an aqueous solution that has a mixture of a weak acid and its conjugate base…

Q: Define a buffer and explain how a buffer works. [Note: be sure to address the role of conjugate…

A: Aspirin is an acetylsalicylic acid synthesized from the esterification of salicylic acid. Salicylic…

Q: How do I prepare a 1 M ammonium acetate buffer with a pH of 7. I have solid ammonium acetate, .1 M…

A: Ammonium acetate buffer: Ammonium acetate buffer can be prepared from the reaction of ammonia with…

Q: Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength…

A: TAE buffer stands for Tris-acetate-EDTA buffer. It is used in lab to do electrophoresis of nucleic…

Q: What is the ratio of TRIS/TRIS-H+ in a TRIS buffer at pH 8.7?

A: TRIS is tris aminomethane. It is an organic compound. It is mostly used as a component of buffer…

Q: If a liquid contains 3 mg of drug in 40 mL, what is the milligram percent of this solution? Round to…

A: Drug calculation is an essential component of drug administration. As Nurses we need to understand…

Q: Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration…

A: The following formula is used to find the volume required to prepare a 5 mL sample with a final…

Q: 50 mL of 15 mg/mL NaOH solution from 100 mg/mL NaOH

A: Sodium hydroxide is an inorganic compound with the formula NaOH. It is an ionic compound consisting…

Q: How would you classify a patient with the following laboratory values? Cr 0.9 mg/dl, BUN 51 mg/dl.,…

A: In azotemia, BUN, creatinine in blood and other secondary waste products are increased in body.…

Q: In water content determination How many mL of water is expected from a 20 g Digitalis sample…

A: A drug, often known as a medicine, is a chemical compound that has a biological effect on the human…

Q: How could you prepare 500 mL of a 6.0 M NaOH solution using solid NaOH pellets? What is the percent…

A: Molar concentration is a measure of the concentration of a chemical species, in particular of a…

Q: How many grams of HCl are needed to make 10 uL of a 0.005g/mL acid solution?

A: Given Values: Volume of HCl = 10 μl Concentration of HCl = 0.005 gml

Q: Tris NaCl buffer is known as?

A: Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. The conjugate acid of…

Q: Why do we need to place the lipid samples under direct heat after adding ethanol in a solubility…

A: The solubility test determines the presence of all lipids as a preliminary test.

Q: What is the pH of a buffer prepared by adding 30.0 mL of 0.25 M acetic acid (CH₂COOH) (K. = 1.7 x…

A: The pH of a buffer prepared by adding 30.0 mL of 0.25 M acetic acid to 125.0 mL of 0.15 M sodium…

Q: A 1.0-L 0.010 M buffer with pH 6.50 is given as an assignment to a group of students. Which is the…

A: A weak acid is used to prevent any change in the pH of the solution, regardless of the solute. On…

Q: how many grams of sr(no2)2 are needed to make 200 ml of 0.300M solution of sr(no2)2

A: Molarity is defined as the number of moles dissolved per 1000 ml of solution.

Q: Calculate the followings:  The pH of the final solution after adding 300 ml of 40 mM NaOH to 600 ml…

A: pH is the scale that is used to specify the acidity or basicity of an aqueous solution. Acidic…

Q: carbonic anhydride

A: Carbonic anhydride It is the acidic oxide of carbonic acid. It is colourless, odourless and…

Q: What is the pH of an aqueous solution with {H3O+} = 6x10-12

A: pH is logarithm of the reciprocal of the H+  or H3O+ ion concentration in a solution that is…

Q: What is the pH of the following buffer mixtures?                                            (a) 100…

A: Given values: (a) Volume of acetic acid = 100 ml Concentration of acetic acid = 1 M Volume of sodium…

Q: What is the pH of a buffer solution made by dissolving 0.10 mol of formic acid, HCOOH, and 0.10 mol…

A: Since a weak acid dissociates partially in an aqueous solution, the magnitude of dissociation is…

Q: How many mL of a ½ strength magnesium sulfate solution will contain 10 grams of magnesium sulfate?

A: Drug dose is the amount of drug in mg which is needed to be administered into the patient  Strength…

Q: 950 mL of 1X CuSO₄ * 5H₂O solution from 25X CuSO₄ * 5H₂O solution

A: Given Values: Stock solution = 25 X CuSO4 . 5H2O Final Solution = 1 X CuSO4 . 5H2O Volume = 950 mL

Q: How much of a 5x buffer and how much water should be added to make 1 L of a 1x solution

A: For application, it's normally diluted to a "1X" concentration. A 5X concentrated solution, for…