How is isotype IgA radioactive decay used?

Q: When would it be beneficial to use a eukaryotic host formolecular cloning?

A: The techniques for  gene manipulation, cloning, and expression were first developed in bacteria.…

Q: What is reverse DNA gyrase and why is it importantto hyperthermophiles?

A: Hyperthermophiles are high temperature loving bacteria. Most common of them is Thermotoga maritima.…

Q: In a conjugation experiment, what is meant by the time of entry? How is the time of entry determined…

A: Conjugation is a method of recombination in bacteria. This occurs by direct connection between cells…

Q: What is the difference between DNA extracted from whole blood and buffy coat?

A: Extraction of genomic DNA from whole blood sample has been a very common practice but the…

Q: Why is it usually more difficult to select recombinants withArchaea than with Bacteria?

A: Archaea are single celled-microorganisms and they are prokaryotes. They comprised of a single…

Q: Why are the recombinants produced from an Hfr x F- crossrarely, if ever, F+?

A: There are many methods which the bacteria used for the transfer of genes from one organism to…

Q: What is a mobilizable plasmid?

A:

Q: In a conjugation experiment, How is the time of entry determined experimentally?

A: Bacterial conjugation is the transmission of bacterial genes via direct contact or by forming a…

Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?

A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…

Q: Why does conjugation occur in bacteria?

A: Conjugation is a process of transfer of genetic material from one bacterial cell to the another cell…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: How is fluorescence in situ hybridization (FISH) used to producea spectral karyotype?

A: Fluorescence in situ hybridization (FISH) is a technique that is used to detect and locate a DNA…

Q: Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase's…

A: Alfred Hershey and Martha chase did so in order to label the protein coat of virus with radioactive…

Q: What is the purpose of the S-9 in the Ames test?

A: The Ames test is a generally utilized technique that utilizes bacteria to test whether a given…

Q: How many different temperatures are used in each cycle of the polymerase chain reaction?

A: Polymerase chain reaction (PCR): It is a molecular technique used to make copies of desired gene.…

Q: What do you mean by Roflumilast ?

A: The activity of the enzyme can be inhibited wither reversibly or irreversibly. Irreversible…

Q: Do the antibiotic-resistant mutants arise in response to the presence of the antibiotic?

A: A mutation is the alteration of the DNA sequence that changes the normal functioning of the DNA.…

Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…

A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…

Q: Why is it essential to add sodium azide to succinate dehydrogenase assay  solution? What is the…

A: In laboratories various assays are carried out for the determination of functionality of various…

Q: How common is dup15q?

A: Dup15q:- Is characterized by having an extra copy of a portion  of chromosome 15[partial trisomy] in…

Q: how Recombinant Plasmid Libraries SimplifyGene Identification?

A: Introduction  Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…

Q: In what ways does conjugation create bacterial cells that are at least partially diploid? Is this a…

A: Conjugation is the process of transfer of genetic material from one bacterium to another by direct…

Q: Why is the catalase test useful for the differentiation of staphylococci from streptococci?

A: Bacteria are classified into two categories gram-positive bacteria and gram-negative bacteria.…

Q: How does a dideoxynucleotide differ from a deoxynucleotide? How are dideoxynucleotides important in…

A: Dideoxynucleotides are DNA polymerase chain-elongating inhibitors used in the Sanger technique of…

Q: What is the pupose of bacterial artificial transformation?

A: Transformation is a process in which the bacteria uptakes the naked DNA into it and incorporated…

Q: How does diagnostic method for detecting Covid-19 using RT-PCR work?

A: RT–PCR also called Real-time PCR (polymerase chain reaction) is a nuclear-derived method or test…

Q: What is conjugation in bacterial cells?

A: Horizontal gene transfer is the process through which an organism incorporates its genetic material…

Q: How is DNA fragmentation test done?

A: Deoxyribonucleic acid (DNA) is the main constituent of the chromosome. It contains all information…

Q: What is the purpose of conducting a modified Furter-Meyer Test? What is the principle behind this…

A: Lipids are the organic biomolecules molecules that are nonpolar solvent and they are composed of…

Q: How does penicillin enrichment make it easier to isolate mutants?

A: Bacteria are a form of a prokaryote that can be found on the surface of the Earth. Some are harmful…

Q: What is the best amount of concentration of DAPI II for counterstaining human chromosomes?

A: DAPI is an approachable nuclear counterstain for use in the multicolor fluorescent techniques. Blue…

Q: How are recombinant plasmids used

A: Plasmids are small,  circular, extrachromosomal, dsDNA presents mostly in prokaryotes and is used as…

Q: What is clinical exome test?

A: The exome is the part of the genome which contain all the protein coding sequences.

Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?

A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…

Q: When is the transformation factor used?

A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…

Q: How is the genome replicated prior to binary fission in a bacterial cell?

A: DNA replication is a process by which duplication of a molecule of DNA takes place. Duplication of…

Q: How Hfr strains allow genetic mapping of the E. coli chromosome ?

A: Bacteria are prokaryotic organism which contains a circular double-stranded chromosomal DNA and…

Q: what are two differences between agarose gel electrophoresis and SDS PAGE?

A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…

Q: How to identify the type of genomic DNA isolation contamination and how to eliminate this…

A: It is chromosomal DNA, in contrast to extra chromosomal DNAs like plasmids. Most organisms have the…

Q: What is the charge of the chromophore/colored ion of the positive staining and negative staining?

A: Introduction The Portion Of A Molecule That Gives It Colour Is Called A Chromophore. Within A…

Q: Why are plasmids used for transformation?

A: Transformation is the genetic alteration in a cell by direct uptake and incorporation of exogenous…

Q: Why is lambda DNA used as a marker?

A: Lambda deoxyribonucleic acid (DNA) is a linear, double-stranded phage DNA containing 12 bp…

Q: What is the radioactive decay process of Isotype IgA?

A: Immunoglobulins are glycoprotein molecules, which are produced by the plasma cells. They act as a…

Q: What is the purpose of conjugation in bacteria?

A: Horizontal gene transfer is the process through which an organism incorporates its genetic material…

Q: What is bacterial conjugation and how was it discovered?

A: Horizontal gene transfer (HGT) is defined as the exchange of genetic material between organisms.…

Q: w to achieve carbon dioxide requirement for capnophilic organisms? What is the used of “Thermus…

A: Capnophiles are microbes that can survive in the presence of higher carbon dioxide concentration.…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.