Why does the nucleus appear deep blue after staining with methylene blue? Is it possible that bacterial cells are also present in the preparation?
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Why does the nucleus appear deep blue after staining with methylene blue? Is it possible that bacterial cells are also present in the preparation?
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- What is the charge of the chromophore/colored ion of the positive staining and negative staining?In Figure 5-5,a. Why do A− and B− cells, by themselves, not formcolonies on the plating medium?b. What genetic event do the purple colonies in themiddle plate represent?When viewing the capsule stain, why do the capsules appear colorless? Explain
- 16:53 In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol The counterstain (safranin) causes the purple color to develop None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The primary stain, crystal violet, is dark purple O LTE O O ×What other method could be used to purify lysozyme from white using its unusual pl?Since higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?
- One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/1) both streak plating and pour plating produce isoluated colonies. What is the underlying explanation for why both methods work; that is, what are both methods doing with repect to the bacterial cells? 2) If streak plates failed to produce isolated colonies, describe two things that you could do to improve your chance of generating isolated colonies. 3) Why do we care so much about producing isolated colonies? what is an isolated colony composed of? What can you do with an isolated colony?You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]