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- How is the information from a Kirby-Bauer disk diffusion test used for the recommendation of the clinical use of an antimicrobial drug?
- Who should be responsible for discovering and developing new antibiotics? Support your answer with reasoning.
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Solved in 3 steps
- What specific medium must be used in testing the effectiveness of antimicrobial drugs?How are dilution susceptibility tests and disc diffusion tests usedto determine microbial drug sensitivity?Could an organism that is susceptible to an antimicrobial agent in laboratory testing fail to respond to it when that drug is used to treat the patient? Explain.
- What is the differece between disinfection and sterilization? What is the dfference between bacterial-cidal and -static antimicrobial agents?In the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria placed on the plate b. type of medium in the plate c. concentration of antimicrobial drug in the disk d. all of theseFor a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. What are the concentrations of antibiotic applied in the test?
- Why are antimicrobials only directly effective against bacteria and what is the control on the antimicrobial plate?What are the major actions of antimicrobial agents? What criteria are used in the selection of antimicrobial agents?Discuss the procedure and application for the agar well diffusion method of evaluating antimicrobial activity.
- Design an experiment to determine whether an antimicrobial agent is acting as a cidal or static effect? How would you determine whether an agent is suitable for use as an antiseptic rather than disinfectant?What is the importance of an antimicrobial removal device (ARD)?Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.