You are studying a drug that leads to cell cycle arrest during mitosis.You examine levels of cyclin and cdk proteins in the cells treated with the drug, anduntreated cells as control.Shown below are the Western blot results of protein expression.DrugNo DrugTime (minutes)153045 60 750.153045 60 75CDKCydinWhich of the following could be a mechanism by which the drug leads to cell cycle,arrest? Which of the following could be a mechanism by which the drug leads to cell cyclearrest?Inhibition of Cyclin synthesisInhibition of CDK synthesisInhibition of CDK-Cyclin activationInhibition of ubiquitination or proteasomal degradation

Name: You are studying a drug that leads to cell cycle arrest during mitosi
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: You are studying a drug that leads to cell cycle arrest during mitosis.You examine levels of cyclin and cdk proteins in the cells treated with the drug, anduntreated cells as control.Shown below are the Western blot results of protein expression.Dru

In the no drug slide, Cyclin disappears at the end because it associated itself with CDK thus…

Science

Biology

You are studying a drug that leads to cell cycle arrest during mitosis. You examine levels of cyclin and cdk proteins in the cells treated with the drug, and untreated cells as control. Shown below are the Western blot results of protein expression.

You are studying a drug that leads to cell cycle arrest during mitosis. You examine levels of cyclin and cdk proteins in the cells treated with the drug, and untreated cells as control. Shown below are the Western blot results of protein expression.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 19P: Figure 28.11 depicts the eukaryotic cell cycle. Many cell types â€œexitâ€� the cell cycle and...

See similar textbooks

Similar questions

"Agent V" is the name of an anticancer (chemotherapy) drug. This drug works against cancer cells by inhibiting the formation of microtubules in sensitive cells. Consider a cell that is sensitive to agent V (in other words, agent V is effective at stopping growth of this cell). Based on this information, agent V would cause the cell to be frozen at which of the major cell cycle checkpoints (G1, G2 or M checkpoint)? Explain
When the DNA of a human cell becomes damaged, the p53 geneis activated. What is the general function of the p53 protein?Is it an enzyme, transcription factor, cell-cycle protein, orsomething else? Describe three ways in which the synthesisof the p53 protein affects cellular function. Why is it beneficialfor these three things to happen when a cell’s DNA hasbeen damaged?
One important biological effect of a large dose of ionizing radiation (like X-rays) is to halt cell division. How does this occur? To answer this question, state the name and role of 2 specific and important proteins involved in the process. What happens if a cell has a mutation that prevents it from halting cell division after be irradiated? Be specific and detailed. An adult human who has reached maturity will die within a few days of receiving a radiation dose large enough to stop cell division. What does that tell you about cell division? In addition, name 2 different/unique cell types that would be primarily affected.
Describe how Ras and p53 can alter the simplified genetic pathway controlling cell division shown below. For each of the two genes, would uncontrolled cell division result from a loss-of-function or a gain-of-function mutation? growth factors - receptors - cyclins - cyclin-dependent kinases - cell division
A cell inherits a mutation in a gene that results in a transcription factor, called NF-kB, constantly being in its active conformation. When active, NF-kB stimulates the expression of cyclins that promote progression of the cell cycle, regardless of other conditions. As a result of this mutation, how would this cell's phenotype be affected by this mutation? A) This cell would have a cancer phenotype B) This cell would grow larger in size, but would never divide C) This cell would likely undergo apoptosis D) This cell would not duplicate its chromosomes .
By what molecular pathway does loss of cell cycle regulation in an organism lead to cancer? What genetic changes can cooperate to accomplish the cancer cell’s escape from the normal balance of cell growth?
When studying living cells in a laboratory, researchers sometimesuse drugs as a way to make cells remain at a particular stage of thecell cycle. For example, aphidicolin is an antibiotic that inhibitsDNA synthesis in eukaryotic cells and causes them to remain inthe G1 phase because they cannot replicate their DNA. In whatphase of the cell cycle—G1, S, G2, prophase, metaphase, anaphase,or telophase—would you expect somatic cells to stay if they weretreated with each of the following types of drug?A. A drug that inhibits microtubule formationB. A drug that allows microtubules to form but prevents them fromshorteningC. A drug that inhibits cytokinesisD. A drug that prevents chromosomal condensation
When studying living cells in a laboratory, researchers sometimesuse drugs as a way to cause cells to remain in a particular phaseof the cell cycle. For example, aphidicolin inhibits DNA synthesisin eukaryotic cells and causes them to remain in the G1 phasebecause they cannot replicate their DNA. In what phase of thecell cycle—G1, S, G2, prophase, metaphase, anaphase, or telophase—would you expect somatic cells to stay if the followingtypes of drug were added?A. A drug that inhibits microtubule formationB. A drug that allows microtubules to form but prevents themfrom shorteningC. A drug that inhibits cytokinesisD. A drug that prevents chromosomal condensation
Part A and B A. What is the normal function of the Rb protein? A) Rb prevents the cell cycle from proceeding unless the cell is prepared to do so B) Rb causes cells to undergo apoptosis C) Rb promotes Cdk activation D) Each of the other selections is true E) Rb repairs damaged DNA B. How do tumors arise? A) When cell proliferation occurs at a higher rate than cell death B) When someone is infected with a tumor from another person C) When cell death occurs at a very high rate D) When cell proliferation occurs at a very low rate
What are the most important steps in cell-cycle regulation? Give some explanations and present some factors leading to uncontrolled cell cycle.
The term heterochromatin refers to heavily condensed regions of chromosomes that are largely devoid of genes. Since few genes exist there, these regions almost never decondense for transcription. At what point during the cell cycle would expect to observe decondensation of heterochromatic regions? Why?
Figure 28.11 depicts the eukaryotic cell cycle. Many cell types â€œexitâ€� the cell cycle and donâ€™t divide for prolonged periods, a state termedG0; some, for example neurons, never divide again. a. In what stage of the cell cycle do you suppose a cell might be when it exits the cell cycle and enters G0? b. The cell cycle is controlled by checkpoints, cyclins, and CDKs. Describe how biochemical events involving cyclins and CDKs might control passage of a dividing cell through the cell cycle.