Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: Why is it important to limit the quantity of cells used to prepare a smear?

A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…

Q: Bacillus cereus divides every 30 minutes. You inoculate a culture with 100 After 3 hours, how many…

A: Bacillus cereus is a rod-shaped, gram-positive bacteria. It is a facultative anaerobe. It is a toxin…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: What is the purpose of flaming an inoculating loop?  How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: Why is Micrococcus luteus a common contaminate on bacteriological media?

A: The strain is typically connected with human skin, especially from the head, arms and legs;…

Q: What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line…

A: An isolated colony represents a pure source of an organism from which a pure culture can be started.

Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?

A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…

Q: Why do you have to boil the agar solution before dispensing it into tubes?

A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…

Q: Why bleach disinfectant will the most effective against probiotic?

A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…

Q: You have isolated a Gram positive cocci from a throat culture. You have determined that it is…

A: Gram-positive bacteria have a single cell wall anchored to the cell membrane by lipoteichoic…

Q: Determine the microbiology tests used to identify the microorganism(cutaneous anthrax) i. Gram…

A: Cutaneous anthrax is the most common form of anthrax infection, and it is also considered to be the…

Q: There are so many microbes in a single mL of culture, it is very difficult to perform one dilution…

A: The microbiology studies about both the diseases causing microbes and beneficiary microbes, about…

Q: Which type of hemolysis characterizes Streptococcus pyogenes, the bacterium that causes strep…

A: BASIC INFORMATION BACTERIA  It is one of the type of biological cell. They belong to prokaryotic…

Q: What color would you expect for a gram positive microorganism for each of his incorrect attempts?…

A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…

Q: Where should a label be written on an agar plate?

A: The aseptic technique is applied in a laboratory setup to reduce the risk of contamination. It…

Q: The below  photograph shows a Gram-stained slide viewed using a light microscope set at brightfield…

A: Gram's staining is a technique used to distinguish between Gram negative and Gram positive bacteria.…

Q: Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to…

A: Microorganisms are defined as organisms that cannot be viewed by unaided eyes and require a…

Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…

A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…

Q: Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood…

A: The chocolate agar and the blood agar media both have the almost same composition. The only…

Q: Why is it important to follow sterile technique?

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?

A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…

Q: PART B Re-watch the video and explain why the following steps are necessary: Why use tweezers to put…

A: Please note as per the site regulations, external links are not watched. Hence, part A cannot be…

Q: Would you use an inoculating loop to transfer bacteria to an agar deep tube?

A: Agar deep tube is a glass tube which is filled with solid medium. The medium is solid due to…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: During media preparation, you observed that the agar medium did not solidify after sterilization…

A: Nutrient Agar Medium is the artificial media which is used to grow microbes. It provides essential…

Q: Which of the following would suggest your stock culture is contaminated? Gram stain indicates…

A: A contaminated culture has had contaminants introduced into it. Contaminants are unwanted microbes…

Q: You collected a sample from a patient with a suspected STI. After Gram staining, you observed this…

A: The staining of bacteria is an important method of classifying the bacteria There could be multiple…

Q: What test can help you differentiate staphylococci from streptococci? What reagent or media would be…

A: Both are gram positive bacteria.

Q: The figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description…

A: METHYLENE BLUE AGAR (EMB Agar) Methylene Blue Eosin Agar is a differential medium used for the…

Q: A laboratory technician performed a series of 10 fold serial  dilutions shown below. he plated 0.1…

A: In this question, we are given colony count from different 10 fold dilutions. The volume plated from…

Q: erial slide agglutination technique important in diagnostic procedure?

A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…

Q: Pretend you want to test whether a certain bacterium can take iron away from hemoglobin. What is the…

A: Culture media  Culture media is a type of media that support the growth of bacteria and fungi and…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: If some cells in your Gram stain are red/pink rods and some are purple cocci, __________.   your…

A: It is a common staining method in microbiology, used to differentiate two large bacterial groups…

Q: am-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…

A: Bacteria are the single celled microscopic organisms. These are can not be seen through the nalked…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate…

A: The first requirement for physically isolating a bacterium is that it can be cultured in the…

Q: Which of the following would typically be the initial indicator of bacteria growing within a blood…

A: Blood agar is one of the commonly used culture methods used for growing micro organisms and growth…

Q: how could you determine if there is any contamination on your agar?

A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…

Q: identifying the type of bacteria I have cultured. I know that it is gram-negative and cocci in…

A: There is only two type of bacterias which do not grow so well on EMB medium.  1. Shigella    2.…

Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…

A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…

Q: With your tests you now figured out that your patient has a Staphylococcus infection, and you would…

A: S. epidermidis and S. aureus come under the category of bacteria and are associated with nosocomial…

Q: You are reading culture plates today and identifying pathogens that grow from patient samples. Which…

A: The culture plates for growing microbes hold importance in understanding and examining the…

Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…

A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…

Q: A mixed sample is collected from a salty environment. Stapnylococcus What is the expected result and…

A: Mannitol salt agar (MSA) is both a selective and differential media used for the isolation…

Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…

A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.