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- Assaying virus concentration requires healthy cell cultures. Give possible reasons to account for you cell culture not having the required degree or confluence at the point they needed for use.The diffraction limit describes the limitationof light microscopes to distinguish objects less than 200 microns apart. Most viruses are less than 200 microns in size. (a) Brefly describe one molecular technique and one microscopy technique that could help you identify the specific virus infecting your patient. Be sure to wxplain how the technique works to identify virus particles. (b) Make an argument for which of the techniques would be best to use during clinical diagnosis.1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above
- Write a lab report on enumeration of lytic viruses : the plaque assay including the sections: introduction, Materials and Methods, Results and Discussion, and citing peer reviewed sources use this data for results section Group Number Number of Plaques Dilution Factor of Plate Original Concentration of Phage 1 58 10^3 5.80E+04 2 37 10^3 3.70E+04 3 44 10^3 4.40E+04 4 63 10^3 6.30E+04 5 36 10^2 3.60E+03 6 65 10^3 6.50E+04 7 260 10^2 2.60E+04 8 39 10^3 3.90E+04 9 178 10^2 1.78E+04 10 192 10^2 1.92E+04 11 274 10^2 2.74E+04 12 224 10^2 2.24E+04 Class Average 3.52E+04Answer the following questions:1. What are the different sections in the Clinical laboratory? Which one you think, will be your favorite?2. Enumerate the 3 Coronavirus strains that caused a large-scale outbreak in humans in the past 2decades.3. What is the first step that should be done in PCR testing?1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?
- Hi, can you kindly make 3 concepts that is related to the research topic which is "Compromising own health care in hospitals due to covid-19 virus. Here's the format below: Concept 1: (Title that is related to the research topic as mentioned above") Definition: (meaning) Description: (Functions, importance, features, effects, disadvantages and advantages) Concept 2 and Concept 3 are same to concept 1's format but each have different title. Kindly put the links of sources tooA new test kit is developed for the direction of coronavirus. When it is tested in a group of 115 patients with Corona virus, 81 have a positive test. In a group of 220 individuals without Corona virus, 10 have a positive test. a. What is the sensitivity and specificity of the test kit? b. Comment on the above results.1. What is the importance of using freshly prepared KOH in testing the bacteria? Explain this comprehensively and please, do not just copy from somewhere.
- 1. Why the virus was not found in the tests? 2. Why the treatments are not working?Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate1. Which of these methods will allow quantification of a vaccine? a. Immunocytochemistry b. Flow Cytometry c. Western Blot d. ELISA 2. Sanger sequencing depends on _____________ during in vitro DNA replication a.random incorporation of fluorescent-labelled deoxynucleotides which releases a color after being added to the DNA chain b.random incorporation of pyrophosphate-labelled deoxynucleotides which a releases color upon addition of substrate c.random incorporation of fluorescent-labelled dideoxynucleotides which terminates the growing chain d. subsequent ligation of a DNA octamer with two known specific bases
