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- What are the difference in the movement of microorganisms under wet mount from hanging drop technique? Provide a descriptive explanation.In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?Could humans exist on chemically defined media for 48 hours? Could you spread plate in the usual Petri dish a 10mL inoculum? Why or why not? In what instances would the pour plate method be more appropriate than the spread plat method?
- Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?The agar turns pink because the enzyme produces blank which causes a ph change to blank?
- Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?Why would hypertonic solution make a potato stick become limper?You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?
- How long does it take for a bacterial culture with a generation time of 12 minutes to increase from 400 cells up to 1600 cells/mL?Which is more Suitable for long-term storage: agar slant or plate? Explain why?If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation