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- Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio) 1 4.47 69.58 How to get the concentration of the mixture in this case?Compare and contrast the amount of caffeine you obtained from the single vs multipleextractions with Red Bull and Coca-Cola beverages. Explain why this has occured. Single Extractions: TEST SOLUTION NAME ABSORBANCE READING [CAFFEINE]mg/100ml 5ml Red Bull 2.412 57.9mg/100ml 5ml Coca-Cola 0.716 16.2mg/100ml Multiple Extractions: TEST SOLUTION NAME ABSORBANCE READING [CAFFEINE]mg/100ml 10ml Red Bull 3.253 78.453 10ml Coca-Cola 0.785 17.844
- A 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to 100.0 mL in a volumetric flask. Aliquots of the sample solution were treated with a lead complexing reagent and water as follows: Solution 1: 10.0 ml blood sample + 20.0 mL complexing agent + 30.0 mL H20. Solution 2: 10.0 ml blood sample + 20.0 mL complexing agent + 26.0 mL H20 + 4.00 mL of 78 ppb Pb2+ standard. The resulting solutions were analyzed by UV/Vis at 375 nm. Absorbance for solution 1 = 0.155 and for solution 2 = 0.216. Calculate the concentration of lead (ppb) in the original sample.You have a concentrated sample solution of a new wine from a winery to analyze for malic acid content. once you have worked up the sample and done one 5x dilution you record an absorbance of .395 at the analytical wavelength of the malic acid. the molar absorptivity coefficient E of the malic acid for this method of measurement is 15.84 mM-1cm-1. What was the molarity of malic acid in original wine sample mM?A standard curve for glucose analysis was prepared.The slope (m) was found to be 1.341 while the y-intercept (b) was -0.333. Calculate the concentration (x) of glucose having an absorbance (y) of 0.151. 0.067 0.361 0.858 0.136
- A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.The concentration of phenol in an aqueous solution is 10 ug/mL. The absorbance is found to be 0.209 when this solution is placed in a 1.00 cm cuvette and 258 nm radiation is passed through Calculate the specific absorptivity, including units, of phenol? What will be the absorbance if the solution is diluted to 5 ug/mL? What will be the absorbance if the path length of the original solution is increased to 5.00 cm?3 to the kit manual 200uL of plasma was mixed with 2.8 ml of the dilution buffer, mixed well then 200 uL of the dilution mixture was pipetted in 0.9 ml of solution A and 0.9 mL of solution B and measured using a spectrophotometer, the concentration was 30. What is the final concentration of the sample after adjusting the dilution factor
- Two components in an HPLC separation have retention times that differ by 15 s. The first peak elutes in 9.0 min, and the peak widths are approximately equal. The dead time to was 65 s. Use a spreadsheet to find the minimum number of theoretical plates needed to achieve the following resolution. R. values: 0.50 0.75, 0.90, 1.0, 1.10. 1.25, 1.50. L75. 2.0 and 2.5. How would the results change if peak 2 were twice as broad as peak 1?You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).60 ml of standard hardness containing 1 mg of pure CaCO3 per ml consumed 22 ml of EDTA. 40 ml of water sample consumed 20 ml of EDTA solution using EBT indicator. 40 ml of water sample after boiling, filtering consumed 15 ml of EDTA. Calculate the temporary and permanent hardness of water sample.
