How to write the methodology? I have a practical of centrifugation, I need to do a lab report, but I don't know how to write the methodology. Here is the introduction: The centrifuge is a common and widely used instrument in the laboratory. In biology, centrifugation is a technique used to separate biological particles or macromolecules for instance cell debris, sub-cellular compone
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
How to write the methodology? I have a practical of centrifugation, I need to do a lab report, but I don't know how to write the methodology.
Here is the introduction: The centrifuge is a common and widely used instrument in the laboratory. In biology,
centrifugation is a technique used to separate biological particles or macromolecules
for instance cell debris, sub-cellular components, proteins,
vaccines, antibodies and etc. Principally, the separation is based on the particle size,
shape, and density where large and high-density particles travel at a faster rate and
will be sedimented first, followed by other particles. There are many different types of
centrifuges that are commercially available such as large-capacity low-speed
preparative centrifuges, refrigerated high-speed preparative centrifuges, analytical
ultracentrifuges, preparative ultracentrifuges, large-scale clinical centrifuges, and
small-scale laboratory microfuges.
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