In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true:     I. Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype   II. Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type)   III. Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
Problem 9P
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In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true:

 

  I.

Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype

  II.

Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type)

  III.

Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype

  IV.

Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to white

vermilion
Wild type
white
rosy
5' P-element end
transposase
inverted repeats
Syncytium
injection
Helper
3' P-element end 5' P-element end
B
white
experimental Selection marker
inverted repeats
Germline
Nucleus
transposase
3' P-element end
Genome
Transcribed Image Text:vermilion Wild type white rosy 5' P-element end transposase inverted repeats Syncytium injection Helper 3' P-element end 5' P-element end B white experimental Selection marker inverted repeats Germline Nucleus transposase 3' P-element end Genome
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