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- What is single guide RNA (sgRNA) ?Describe the process of RNA splicing, including the roles of the spliceosome and self-splicing RNA molecules.A double-stranded RNA has been generated by release/splicing out of an intron within the nucleus...outline the pathway taken to generate microRNA and its action on the shutdown of protein biosynthesis
- What is RNA modification? Explain with the help of diagramsmiRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might identify potential mRNA targets for a given miRNA if you only know the sequence of the miRNA and the sequence of all mRNAs in a cell or tissue of interest.. Eukaryotic processing of hnRNA into mature mRNA includes all of the following steps except: ribosome attachment of methionine to the 5’-AUG-3’ codon 5’-addition of a 7-methylguanosine cap 3’-addition of a polyadenylated tail splicing together of exons excision of introns
- Why are miRNAs called noncoding RNAs? Explain how they participate in gene regulation.A gene contains eight sites where alternative splicing is possible. Assuming that the splicing pattern at each site is independent of that at all other sites, how many splicing products are possible?What makes the bacterial and eukaryotic initiator tRNAs different from other RNAs, and how do these differences help them carry out initiation rather than elongation?