If a fluorescent spot appears on a microarray, whatinformation does this provide regarding gene expression?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: What is the general effect of methylation of a genomics region on transcription?

A: DNA methylation is a biochemical process involving the addition of methyl groups to the Dna strand.…

Q: What is a forward genetic screen?

A: Genetics is a division of biology that includes study of heredity, genes and genetic variation.…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?

A: Genome editing tools are those tools which help in making changes into the genome of an individual.…

Q: How to do linear Restriction Mapping of gene fragments, including a combination of three enzymes?

A: Bacteria contain restriction enzymes. To destroy viruses, bacteria use restriction enzymes, which…

Q: From your knowledge about DNA microarray, answer the following: Mention the name and the color of…

A: DNA microarray is basically a collection of microscopic DNA spots that are attached to the solid…

Q: Comment on the utility of variability in number of tamndem repeats during DNA fingerprintingds?

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: Does use of nucleic acid probes depend on gene expression?

A: Introduction: Nucleic acid hybridization or nucleic acid probe are used to determine specific gene…

Q: Why Gene-prediction programs are used ?

A: In a DNA sequence, where those codons might fall even where a functional protein might be present or…

Q: How Linker scanning mutations identify transcription control elements. ?

A: The regulation of gene expression is dependent on many factors. The promoter is the upstream…

Q: What is a Paralog and How do you identify a paralogous gene?

A: A gene is a unique sequence of nucleotides which forms a part of a chromosome, which determines the…

Q: How is methylation protective against restriction endonucleases?

A: DNA methylation is an epigenetic mechanism where a methyl group is transfered onto C5 position of…

Q: What is hierarchical sequencing?

A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…

Q: What would happen if the restriction enzymes do not cut the DNA at specific recognition sequences?

A: Restriction enzymes are the enzymes that cut DNA at specific sites in order to make short fragments.…

Q: What detection method could be used to determine the mutagen added to a microbial culture resulting…

A: The mutagens are chemical mixtures or a type of radiation that cause irreversible and heritable…

Q: Besides gene expression, what else can be assayedusing gene chips?

A: DNA microassays or DNA chips contain thousands of DNA sequences of individuals arrayed at a great…

Q: What is the relevance of the sample size to the choice of test organisms for genetic studies?

A: The sample size is the number of organisms chosen for a study or experiment. It can be large or…

Q: How were the specific sequences of triplet codes determined experimentally?

A: The DNA genetic code comprises a set of rules and instructions to obtain proteins and molecules…

Q: What are the basic differences between prokaryotic and eukaryotic regulation of gene expression?

A: Introduction - A single prokaryotic cell makes up a prokaryote. In plants, animals, fungi, and…

Q: How Dolly Sheep was cloned?

A: Cloning was the most important discovery in the history of science. The dolly sheep was the fist…

Q: Why are temperature-sensitive mutations useful for uncovering thefunction of a gene?

A: Mutations are alterations in the DNA sequence due to the mistakes during cell division, exposure to…

Q: What is the most efficient way to regulate gene expression from the perspective of cellular…

A: Gene expression is the process by which the instructions stored in the DNA sequence of genes is used…

Q: How to identify all the somatic mutationswithin tumors ?

A: A somatic mutation is a modification within the desoxyribonucleic acid sequence of a somatic cell of…

Q: Vhat is the main difference between eukaryotic and prokaryotic gene regulation?

A: As prokaryotic organism lack nucleus, DNA (Deoxyribonucleic acid) floats freely in this single…

Q: What is restriction fragment length polymorphism and which method do we use to detect it?

A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…

Q: How is DNA microarray technology used to determine the differences in gene expression?

A: A Deoxyribonucleic acid (DNA) microarray is a collection of microscopic DNA spots attached to a…

Q: Why is it useful to survey expression of the entire genomeunder particular conditions?

A: Expression of the entire genome involves studying expression and the variation in expression in a…

Q: What is a restriction fragment?

A: A restriction fragment can be defined as the amount of  DNA fragment which occurs due to the cutting…

Q: What is the utility of a reporter gene?

A: Introduction Reporter gene are the genes which helps in detection of transformant cell as their…

Q: What is meant by a transgenic animal?

A: Recombinant DNA technology involves integrating DNA molecules from two different species and…

Q: Positional cloning identifies DNA______________s that arelinked to disease genes.

A: DNA or deoxuribonucleic acid is a polymer of deoxy ribonucleotides connected together via…

Q: What do microarrays tell you that studying gene expression byassaying individual enzymes cannot?

A: Introduction  Gene are the key component of genetic material which control almost all the cellular…

Q: How many restriction fragment length polymorphisms are there in the humangenome?

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: What is a transgenic plant?

A: Introduction  Genes are the key components which plays major role in controlling almost all the…

Q: How are different variable region sequences generated?

A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…

Q: When measuring gene expression, what is RNA abundance?

A: Answer: Introduction: RNA molecule consists of four nucleotide bases these are adenine (A), cytosine…

Q: What is a mutagen?

A: The human genome is very dynamic in nature, it tends to mutate and any change in the genetic…

Q: What are the roles of the restriction enzyme and selective plating in cloning a gene?

A: Introduction :- DNA-cutting enzymes are restriction enzymes. Each enzyme detects a single or a few…

Q: What is microsatellite polymorphism?

A: Polymorphism:   It is set place in population when two members or at least two or more members of…

Q: For a translational reporter, is GUS/lacZ the best option, or would other reporter genes provide…

A: Ans  - The LacZ gene from E. coli is frequently used as a reporter gene because it produces a blue…

Q: For what the first clinical gene therapy was given?

A: Gene therapy is a medical technique in which genes are used for treating and preventing a disease.…

Q: What parts of the genome are reversibly methylated? How does this affect gene expression?

A: DNA methylation is a biological mechanism in which methyl groups are introduced to the DNA molecule.…

Q: What is the Ames test and how is it carried out? What assumption concerning mutagenicity and…

A: Mutagens are chemical compounds that cause changes in the DNA. These changes are irreversible and…

Q: compare oligonucleotide arrays and expression arrays

A: An oligonucleotide array is made up of a collection of short single-stranded DNA sequences…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.