What is a read (Sanger sequencing)?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: What is a closed genome?

A: A pan-genome is also called as supragenome, which is the entire set of genes for all strains of…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: What is a genomic library?

A: Deoxyribonucleic acid (DNA) is a molecule composed of two polynucleotide chains. It coils around…

Q: How are the repeated sequences generated?

A: Introduction Repeated sequences are patterns of nucleic acids (DNA/RNA) that occur at multiple sites…

Q: What do the spacers within the CRISPR region correspond to?

A: CRISPR that is clustered regularly interspaced short palindromic repeats. It is found in the…

Q: Who proposed shotgun sequencing?

A: The method used for the sequencing of random DNA strands is genetics, shotgun sequencing.  This is…

Q: what is a palindromic sequence?

A: If a nucleic acid sequence in a double stranded DNA or RNA molecule is read in a particular…

Q: does Sanger sequencing still used?

A: Sanger sequencing is a first generation DNA sequencing method. Despite the advantage of next…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: What is exome sequencing ? Why it is important ?

A: The exome has historically been described as the sequence in the genome that includes all exons of…

Q: What does SDS–PAGE stand for? What is the benefit of doing SDS– PAGE?

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: What is RNA sequencing ? Why it is important ?

A: The biochemical material that is carried from the preceding generation to the succeeding generation…

Q: What does whole exome sequencing reveal?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: What is the principle behind to Sanger sequencing?

A: Sanger sequencing is a method of DNA sequencing, which is based on selective incorporation of…

Q: What are the two methods used for sequencing in Human genome project.

A: Answer- Human genome project was the mega project that aimed for sequencing of whole human genome.

Q: What is the significance of mapping the human genome?

A: Human genome : It is a complete set of nucleic acid sequences for humans , encoded as DNA within the…

Q: Approximately how many base pairs were identified in the human genome working drafts?

A: The human genome project is a scientific project led by James Watson and Francis Collin, started in…

Q: Define about Sanger sequencing ?

A: Introduction:- The process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: What is meant by the phrase reading frame?

A: The genetic sequence is comprised of nucleotides, which are joined together to form polynucleotides…

Q: What is a open genome?

A: Open genomes main aim is to provide enhance basic research , improve public health by combining the…

Q: What

A: INTRODUCTION:- Shotgun sequencing involves randomly breaking up DNA sequences  into lots of small…

Q: What are gene coordinates?

A: Genes are the basic structural and functional units of heredity. They carry coded genetic…

Q: How are different variable region sequences generated?

A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…

Q: Why is GenBank important and What is GenBank format?

A: GenBank is the Genetic sequence database at NCBI (National center for biotechnology information). It…

Q: what is   Inverse PCR ?

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: What is the Kozak Sequence?

A: A small amino acid sequence act as an initiation sequence and binds to the mRNA in order to start…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: Why does reversible chain termination sequencing yield short reads?

A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…

Q: What is a segmented genome?

A: The complete genetic information of an individual is called a genome.

Q: What are steps of sequencing?

A: Introduction: Considering that it is DNA sequencing.  DNA is the genetic material of a living cell.…

Q: What is the Pan genome  ?

A:

Q: Hi, I would like to know which program is used for the graphical presentation of the results of a…

A: A genome wide linkage association study (GWAS) is an approach used in genetic research to associate…

Q: Explain how Sanger sequencing works

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: What is meant by sequencing by synthesis?

A: Genome sequencing is the technique that involves the generation of genetic information found in DNA…

Q: What is a microarray? How can it be used to obtain information about gene function?

A: A gene is a specific nucleotide sequence in RNA or DNA. It is generally located on a chromosome.…

Q: What can exome sequencing do for you?

A: The sequencing of the nucleic acid is the process through which the exact sequence of the nucleotide…

Q: How would one recognize a gap in the genome sequence following nucleotide sequencing?

A: DNA is made up of four bases namely adenine (A), thymine (T), cytosine (C), and guanine (G). The…

Q: What is the largest genome?

A: Genome is the genetic material of an organism. It consists of DNA. Genome is of two types- Nuclear…

Q: What key molecules are essential for Sanger sequencing?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: What does genome sequencing tell you?

A: Genetics is the branch of biology which deals with genes, heredity, and genome in the organism.…

Q: What is the significance of digesting the 16S rRNA product in 16s gene sequencing?

A: The 16S rRNA is a structural component of the 30S ribosomal subunit of  bacteria and archea.

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.