Q: What is the natural habitat of E.coli?
A: In this question we will discuss about the natural habitat of E.coli.
Q: A genetically engineered microorganism used successfully in bioremediation of oil spills is of which…
A: A microorganism or microbe is a microscopic organism which may exist in its single-celled form or in…
Q: Bacterial biofilm may link to
A: Answer: Biofilms are the complex structure composed by the various microbes such as bacteria , fungi…
Q: What is the definition of the term bioremediation? Which elements can a bacterium obtain from…
A: Pollution is when harmful contaminants enter the environment. Remediation involves removal of the…
Q: Why do you think bacterial diseases are becoming more widespread in recent years?
A: In this context, one of the main reasons is the misuse of antibiotics in general. Remember that…
Q: Why is there a need for an incubator in the lab? How about incubating cultures at room temperature?…
A: The Incubator is an insulated device in which the conditions like temperature, humidity, pH, and…
Q: Why are endospore forming bacteria commonly found in soil?
A: A bacteria is a cell which is susceptible to a variety of environmental stress. Any environmental…
Q: Which of the following bacteria can survive in in temperatures ranging from 390F (40C)---to 990F…
A: Thermophiles Are a type of heat-loving microbe that lives in a variety of ecological niches such as…
Q: How did Escherichia coli become pathogenic?
A: Escherichia coli or E. coli can be described as the gram negative, facultative anaerobic, rod shaped…
Q: Why are meats prime vehicles for pathogenic E. coli? How cancontaminated meat be rendered safe to…
A: E.coli is a gram-negative bacteria. It is a facultative anaerobe. Has a rod shaped structure and…
Q: Why do we test water for coliforms if they are not considered pathogenic?
A: Introduction Coliforms are gram-negative, non-sporulating and motile or immotile bacteria. They…
Q: Which of the following bacteria possesses a spherical shape? a) E. coli b) Bacillus anthracis c)…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: Are coliform bacteria dangerous in the water?
A: Coliforms are bacteria that are always present in the digestive tracts of animals, including humans,…
Q: Why are coliforms better indicator of salmonella rather than E. coli?
A: It is considered that Salmonella is best detected by coliforms rather than E. coli. The reason is…
Q: Do you think scientists working at Pitch Lake were at a great risk ofinfection from the organisms…
A: Pitch lake located on the Caribbean island of Trinidad and Tobago. It is a poisonous, toxic,…
Q: Explain the Agrobacterium ?
A: Ans: Agrobacterium: These are Gram-negative organisms which are discovered by H.J. Conn
Q: How does the E. coli strain 026:H11 damage its victims?
A: The enterohaemorrhagic E. coli (EHEC) serotype O26 is the second most common enterohaemorrhagic E.…
Q: Which of the two carbon sources, glucose or acetate, is more advantageous for the cultivation of…
A: E.coli - Escherichia coli, it requires a carbon source to grow to serve as substrate for the…
Q: what is e-coli. How is it treated?
A: In Microbiology, various microbes influences the life of a living individual.The microorganisms…
Q: How might the work of Winogradsky and Beijerinck have contributed to research on bacterial…
A: Winogradsky was primarily interested in soil bacteria involved in nutrient cycling, specifically…
Q: Bacillus subtilis and what enviroments does it thrive in and why?
A: Bacillus subtilis has been one of the most commonly employed species in the study of genetic…
Q: why the vibrio bacteria might survive better in colder temperatures? what part of their DNA or…
A: There are several stimulus-response pathway which help Vibrio to survive better in colder…
Q: Gram negative bacteria typically display greater antibiotic resistance than gram positive bacteria…
A: Question- Gram negative bacteria typically display greater antibiotic resistance than gram positive…
Q: Which bacterium reduces nitrates in soil to nitrogen?
A: Nitrogen is needed by the organism for producing amino acids. Atmospheric nitrogen cannot be used as…
Q: How long is the generation time of E coli?
A: Bacteria are prokaryotic organisms.
Q: Why has it been so difficult to associate human disease, orhealth, with changes in the gut microbial…
A: Step 1 Gut microbial community is defined as the totality of microorganisms, bacteria, viruses,…
Q: What are THREE indications of a high coliform counts in a food product?
A: The coliform count is an indicator of hygiene and a high level of coliform generally depicts…
Q: Which type of food had the lowest bacterial count? Explain.
A: Introduction: Bacterial count is an important factor to assess in the prepared food. It gives an…
Q: what type of bacteria may cause spoilage in coconut water that has been in the fridge for more than…
A: Coconut Water Coconut water (CW) is a slightly sweet liquid made from the endosperm of coconuts.…
Q: tists have cultured bacteria isolated from within frozen mammoths, which are thousands of years old.…
A: Microbes from thousands of years ago have been cultured from early fossils such as frozen mammoths.…
Q: Where do the different types of coliforms come from?
A: Coliform are the rod shaped, gram negative bacteria, used as indicator organism for quality check of…
Q: List five characteristics of coliform bacteria.
A: Coliform bacteria belong to Enterobacteriaceae family.
Q: If the bacterium in question during the perlo outbreak had beenE. coli instead of S. aureus, how…
A: The perlo outbreak was caused by Staphylococcus aureus that causes food intoxication and had led to…
Q: Which bacterium helps in dairy industry?
A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…
Q: Does the presence of coliforms in water sampe indicate that pathogenic organisms are present
A: Coliform bacteria are well-known markers of hygiene, water quality, and food safety. They're…
Q: Why is it important to distinguish between E.coli & E. Aerogenws in contaminated water samples?
A: E.coli is a rod-shaped, gram -ve, facultatively anaerobic, coliform bacterial species that is…
Q: ive two reasons why E. coli is naturally resistant to antibiotics like Penicillin?
A: Bacterial survival in an adverse situation, in a substrate is given as the ability of bacteria to…
Q: Of what benefit is the organism Oxalobacter to human health?
A: Bacteria are microscopic single-celled prokaryotes that thrive in diverse environmental conditions.…
Q: Which type of food had the highest bacterial count? Explain.
A: Bacteria consist of all the characteristics of a living being and can reproduce independently via…
Q: Why is most decomposition typically anaerobic in the deep waters of lakes and oceans?
A: Decomposers are mainly bacteria and fungi are part of the food web. They break down dead organic…
Q: Why do petroleum-degrading bacteria need to attach to thesurface of oil droplets?
A: Petroleum is a crucial strategic resource and all countries compete fiercely for it. The…
Q: Bacteria living in the colon have what type of 'relationship' with their human host?
A: Answer: A parasitic relationship is one in which one organism, the parasite, lives off of another…
Q: Explain why the total bacterial acceptable levels are higher than the coliform acceptable levels?
A: The number of bacterial-colonial units present in the sample is a total bacterial (TBC), which…
Q: Which bacteria is a common cause of food poisoning?
A: Answer: FOOD POISONING : It is the condition when the disease is caused by ingestion of the spoiled…
If coliform bacteria are native to human colons, why the big concern over coliform contamination?
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- For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?Using conversion factors, solve each of the following clinicalproblems:a. A nurse practitioner prepares an injection of promethazine,an antihistamine used to treat allergic rhinitis. If thestock bottle is labeled 25 mg /mL and the order is a doseof 12.5 mg, how many milliliters will the nurse draw up inthe syringe?b. You are to give ampicillin 25 mg/kg to a child with a massof 62 lb. If stock on hand is 250 mg /capsule, how manycapsules should be given?If the data from an enzyme experiment is plotted as a Lineweaver-Burk plot, and the Vmax is 0.02 mol/sec, and x-intercept is –2.5 mM then what is the KM value? Show yourwork/reasoning.
- The Lineweaver-Burke plots of a reaction without inhibitor and one with non-competitive inhibitor will have the same 1. Vmax2. Km3. Km/Vmax4. 1/VmaxYou perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.How does one measure Km using a Lineweaver-Burk plot? 1. By determining the reciprocal of the y-intercept 2. By determining the negative reciprocal of the x-intercept 3. By determining the slope and multiplying it by kcat 4. By determining the saturation point
- The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below. Time (min) Concentration (μM) 0.5 6.01 1 11.78 1.5 17.6 2 23.51 2.5 29.58 3 35.31 3.5 39.73 4 44.5 4.5 48.18 5 50.05 5.5 52.72 6 54.01 6.5 55.06 7 55.65 7.5 56.39 8 56.74 8.5 57.49 9 58.03 9.5 58.61 10 58.69 Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R2 value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate. To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear…Using conversion factors, solve each of the following clinical problems:a. The physician has ordered 1.0 g of tetracycline to be given every six hours to a patient. If your stock on hand is 500-mg tablets, how many will you need for one day’s treatment?b. An intramuscular medication is given at 5.00 mg / kg of body weight. What is the dose for a 180-lb patient?c. A physician has ordered 0.50 mg of atropine, intramuscularly. If atropine were available as 0.10 mg /mL of solution, how many milliliters would you need to give?d. During surgery, a patient receives 5.0 pt of plasma. How many milliliters of plasma were given?Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280 nm. The sample was diluted (200 μL of purified antibody in 800 μL buffer) prior to analysis using a spectrophotometer. Calculate the concentration of antibody in the purified fraction if the Abs=0.95 of the diluted antibody. The molar absorptivity is known to be 191,411.6 M-1cm-1 and the molecular weight is 150 kDa. The pathlength for the cuvette is 1 cm.
- The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?Table of caffeine standards concentration Sample Conc, ppm Std1 16 Std2 32 Std3 48 Std4 64 Std5 80 If the volume used to make 100 mL of std 1 is 2 uL what is the concentration in M used to make a standard calibration curve? The standards are going to be used to build calibration curve to analyze caffeine in an energy drink. If 500 mL of the energy drink has target of 400 mg caffeine, how will you prepare the sample if you need 10 mL for the analysis ? . Caffeine MM=194.19 g/mol.Salt Concentration (%) Transmittance (%) 0 77.23 3 79.23 6 80.39 9 82.71 12 85.66 15 87.55 What is the dependent variable and the independent variable? what patterns do you observe based on the information in the table?