How does one measure Km using a Lineweaver-Burk plot? 1. By determining the reciprocal of the y-intercept 2. By determining the negative reciprocal of the x-intercept 3. By determining the slope and multiplying it by kcat 4. By determining the saturation point
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How does one measure Km using a Lineweaver-Burk plot?
1. By determining the reciprocal of the y-intercept
2. By determining the negative reciprocal of the x-intercept
3. By determining the slope and multiplying it by kcat
4. By determining the saturation point
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- For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.Salt Concentration (%) Transmittance (%) 0 77.23 3 79.23 6 80.39 9 82.71 12 85.66 15 87.55 What is the dependent variable and the independent variable? what patterns do you observe based on the information in the table?During transfer of liquids using a micropipette: What would it mean if the mean is very close to the expected value but your standard deviation is high ?
- - Why Michalis plot is not used, instead double reciprocal plot is used to determine KM and Vmax?what are the complications or the low-reliability in determining the value of KM using graphical methods from a Michaelis-Menten PlotThank you for helping me. I have to questions. In solution B, if we choose two other points on the graph for calculating the slope, wouldnt the values for Km/Vmax be different than 6.1? Why did you choose exactly those points on the graph? Another thing, I understand the method you used to get to Km/Vmax= slope, but how did you then get the value for Vmax to be 10 mM/s??
- Please discuss the value of international normalized ratio (INR) as a test. Why do you think this is more reliable that the ordinary PT.What is the purpose of checking the %GC in our NGS data? What is the ideal %GC value? Explain why this is the ideal value.PLease help me with then following and explain. make sure your asner is correct pls and thank you. If you increase the sample size (n) of a sample, will the following values increase, decrease, remain similar, or remain exactly the same? A) Y ̅ B) σ C) s D) SE_Y -
- Plot a line weaver-burk graph for Km=6.30mM and Vmax=360uM/min when an experiment has 5 tubes with concentrations of substrates of 1.0mM, 10mM, 50mM, and 100mM.In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the amount of cholesterol in the serum sample is determined as 3.11 g/L, what is the absorbance value of the sample? A. 0,280 B. 0.028 C. 0.208 D. 0.82