If conjugation has occurred between the provided E. coli strains I and II, what might we expect to see in the resulting bacteria (i.e., the recombinant)? Select ALL that are feasible. Hint: Consider the possibility that extrachromosomal genes (i.e., on the plasmid) have been transferred or genes originally present on the bacterial chromosome (indeed possible!) have been transferred. A. Recombinant bacteria that are resistant to Amp and Str B. Recombinant bacteria that are resistant to Amp and Nal C. Recombinant bacteria that are resistant to Nal and Str D. Recombinant bacteria that are resistant to Str only E. Recombinant bacteria that are resistant to Nal only My answer is only A. Is my answer is correct?

If conjugation has occurred between the provided E. coli strains I and II, what might we expect to see in the resulting bacteria (i.e., the recombinant)? Select ALL that are feasible. Hint: Consider the possibility that extrachromosomal genes (i.e., on the plasmid) have been transferred or genes originally present on the bacterial chromosome (indeed possible!) have been transferred. A. Recombinant bacteria that are resistant to Amp and Str B. Recombinant bacteria that are resistant to Amp and Nal C. Recombinant bacteria that are resistant to Nal and Str D. Recombinant bacteria that are resistant to Str only E. Recombinant bacteria that are resistant to Nal only My answer is only A. Is my answer is correct?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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After digesting the DNA produced by PCR (generated using the primers mentioned in question 2 added for context below) and the pET-28a vector with restriction enzymes SacI and HindIII, what steps would be needed to obtain a pure culture of E. coli cells that contain the pET-28a-OXA-M290 plasmid (i.e., a culture which consists only of E. coli cells that contain this plasmid)? And, how could you confirm that the OXA-M290 gene was successfully cloned into the pET-28a vector without any mutations? [4 – 8 sentences suggested] Question 2 (Do not answer, just for context):The forward primer that will be designed is the primer that binds to the region of the OXA-M290 gene where the start codon is located, and the reverse primer is the primer that binds to the region of the gene where the stop codon is located. In order to clone the PCR product generated using these primers into pET-28a, extra nucleotides must be added to the 5' ends of the primers. These extra nucleotides will contain the…
In relation to the use of restriction enzymes in recombinant DNA technology, answer the following: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different plasmid, now you do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in Figure below. You have the opportunity to test just one sample from one of your tubes. By utilizing agarose gel electrophoresis technique, which restriction enzyme OR combination of restriction enzymes would you use in this experiment to determine which plasmid is found in which tube?. (Hint: if you use Hind III restriction enzyme you are going to get ONE single fragment with a molecular size of → 0.5+0.3+0.2+0.4+1+1 = 3.4 kb).
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