Please read the case file from exercise 21 (Catalase, pg. 127) in your manual. After reading the case file please answering the following:6. If the result of the catalase test came back positive and the doctor decided to run a coagulase test what do you think the result would be and why? Explain. Microbial Nutrition, Ecology, and GrowthEXERC21Oxygen and Growth: CatalaseCASE FILEPatient G is a 50-year-old woman. She developed several intraab-dominal abscesses following surgery for a kidney transplant.Treatment with IV ampicillin and gentamicin was not effective.The patient's temperature and white blood cell count bothcontinued to be elevated. Afterabdominal CT scan, theabscesses were drained and samples were sent for testing.Gram stains and blood cultures were performed at the sametime, and both came back positive for gram-positive cocci. Further test-ing is under way to identify the organism. Treatment with vancomycinhas been initiated pending the results of the further testing. A consulta-tion with a clinical pharmacist was requested.OChris Ryan/Getty Images RFLEARNING OUTCOMESAt the completion of this exercise, students should be able to• understand the function of catalase in cells that producethe enzyme.perform and interpret the results of a catalase test and knowtheir value in differentiating bacteria.BackgroundAs the bacteríal cell carries out different metabolic pathways, a harmfulby-product called hydrogen peroxide (H,O2) is produced. Hydrogenperoxide is produced by the electron transport chain during the reductionof oxygen. Oxygen acts as the final electron acceptor in the process ofaerobic respiration. Enzymes of the electron transport chain called oxidasesreduce oxygen to form, among other products, hydrogen peroxide (H2O2).H2O2 is considered a reactive oxygen species (ROS) and can causesignificant damage to important molecules of the bacterium like DNA,RNA, and proteins. To detoxify this ROS, many bacteria produce theenzyme catalase. The function of catalase is to convert H,O2 into H20and O2 gas, which are harmless to the cell.2 H202 + 2 H,0 + 02By detoxifying H,O, catalase allows the bacterium to survive andthrive in oxygen-rich environments.To determine if an organism produces catalase, an inoculum is placedon a glass slide. A few drops of H2O2 are then dispensed onto the cells.

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The catalase test is used to distinguish various bacteria types. For instance, the morphologically…