if you are running quantitative test where serum is diluted 6 times and you observed agglutination till the last dilution what does that mean? Does there a specific number for antibody titre?

Q: n western blotting, secondary antibody is conjugated with color or fluorescence producing enzyme…

A: In recombinant DNA technology, several group of techniques are involved. These techniques are…

Q: In (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s…

A: In direct method of antigen detection, the primary antibody is directly attached to a label. It…

Q: What is the significance of a rise in titer of specific antibodies in serum samples taken at…

A: Antibodies or immunoglobulins are the Y-shaped molecules that are responsible for binding to the…

Q: Counterstaining with Hematoxylin and Eosin is an important step in which of the following…

A: Counter stains are stains that are that are in different contrast color to that of the primary…

Q: What is the enzymatic tag on the antibodies that interacts with the substrate causing the color…

A: Enzyme linked immunosorbent assay( ELISA) is biochemical analytical assay used for the detection of…

Q: What does blood typing detect?       presence of surface feature molecules in the blood anti-sera…

A: Using the method blood typing, the universal ABO blood group system has been established.

Q: If 0.9 ml. of a vaccine contains 1595 units of antigen, how many units would be present in each…

A: The antigen is referred to as the substance that is principally accountable for generating an immune…

Q: How would you determine the optimal antigen: antibody ratio by means ofthe ring test?

A: Serology is a branch of medicine that deals with utilization of the blood samples in the laboratory…

Q: What is the principle of Immunochromatographic system, and its uses? Long answer please

A: Answer: CHROMATOGRAPHY TECHNIQUE = It is the technique which is used to separate the components of a…

Q: Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold…

A:

Q: 2) Now you would like to raise antibodies to this protein of YFG. How could you use the pure protein…

A: Antibodies generate by the immune system in body against the antigen which is foreign particle…

Q: The antibody region that bind the antigen region with a high affinity is called: complementary…

A: Antibody, also called as immunoglobulin, is a protective protein produced by the immune system in…

Q: You are testing 950 people with your innovative Covid-19 test. In your test the number of person…

A: The disease is an abnormal state caused due to the deviation to the normal. The disease may be…

Q: In this diagram, why is anti-human IgG used in the indirect test but not in the direct test?

A: An antibody is the Y-shaped molecule that detects a particular antigen specifically. An antigen has…

Q: What is the result of the Antibody Screening Test? Based on the result of the autocontrol, it is…

A: Answer :- Lewis antibody are naturally occurring antibodies almost IgM type found almost exclusively…

Q: What is the point to labeling a secondary antibody with a marker that can be visualized instead of…

A: Introduction: The antibodies are used to detect certain antigens in a technique called ELISA. In…

Q: n not more than 100 words, What antigens/strains are used for the Weil-Felix test, and from what…

A: Well Felix test is an agglutination test for the diagnosis of Rickettsia infection. The Well Felix…

Q: how does teh ELISA  help determined who the initial disease carrier is.

A: The enzyme linked immunosorbent assay is an analytical biochemistry assay which uses a solid phase…

Q: A positive ELISA result with a negative control is called a: A.false positive   B. false negative

A: A negative control is a control group in an experiment that uses a treatment that isn't expected to…

Q: be 96% sensitive. What does that mean? A western blot was used on the same sample to confirm, and…

A: Enzyme linked immunosorbent assay is a very sensitive test used to detect and also quantify…

Q: Based on the given picture, answer the following questions (5 ,6 and 7). How will you describe…

A: Immunodiffusion is the process in which antigen precipitates with an antibody inside an agar gel as…

Q: 1.  Serial dilutions are made in order to determine the level of the antibody in the sample.   True…

A: Q1 has been answered here as per the policy of Bartleby. Please repost the second question…

Q: why do we have to do a western blot confirmatory testing after doing an ELISA screening test for…

A: The human immunodeficiency virus causes the chronic virus infection known as acquired…

Q: Write down the biomarkers and their importance in cbc test?  Explain at your own words

A: Complete blood count test It is the test to perform to evaluate various parameters of blood cells.…

Q: Why monoclonal antibodies method is better than Polyclonal Antibodies method in detecting specific…

A: Y-shaped immunoglobulins are called antibodies which are produced by B cells. When a foreign antigen…

Q: Which technique is best for determing the the intracellular localization of a protein? Question…

A: Intracellular localisation of protein is the determination of the cellular components where protein…

Q: If you are working in a diagnostic lab, what will be your choice among Monoclonal or Polyclonal…

A: Antibodies can be defined as the type of Y-shaped proteins which is part of the adaptive immune…

Q: why would there be some additional bands on an immunoblot that do not match the molecular weight of…

A: Immunoblot or western blot is a technique where we can detect the presence of a particular protein…

Q: RESULTS OF BLOOD TYPING OF “TYPE B+”   Reaction to Anti Serum A Reaction to Anti Serum B Antigen…

A:

Q: After the addition of the 2nd Antibody, how did you determine which wells contain the Antigen bound…

A: The ability of the antibody to bind antigens is used in the immunological assays to identify the…

Q: What is the principle behind Liebermann-Burchard test? And What is the clinical significance of…

A: Liebermann-Burchard test used for estimation of unsaturated steroid (cholesterol) and…

Q: In which type of ELISA antigen exist in between the two antibodies?     Direct     Indirect…

A: Answer of the question given below...

Q: What is the process in which antibodies attach to antigens, causing the formation of masses of…

A: Because the Y-shaped antibody arms randomly attach to many surfaces of non-self red blood cells,…

Q: labeled secondary

A: Antibodies are the proteins (immunoglobins) produced by B-Lymphocytes. There are two groups of…

Q: The primary antibody detects the antigen of interest. What does the secondary antibody detect? If…

A: Antibodies are the bio-molecules which are also known as immunoglobulin and they are produced by…

Q: If a blood sample agglutinates when mixed with Anti-A serum, but does not agglutinate when mixed…

A: Agglutination test is the test which is used to find the blood group with the idea of coagulation.…

Q: When a mother passes antibodies through her breast milk to her baby this is:       Natural…

A: The condition that permits either natural or artificial resistance to particular disease is said to…

Q: What are the two fluorescent compounds that can be used in antibody labelling?

A: Immunoassays make use of a wide variety of antibodies to detect and quantify antigens. In these kind…

Q: What is HIV?  Discuss what an immunochromatographic assay is  If a positive band appears in the test…

A: The Human Immunodeficiency Virus (HIV) is a virus that causes infection in humans. It is a virus…

Q: What p-value is associated with t-1.03 and n=26 for a 1-sided t-test? O p=0.15 O 0.10 > p > 0.05 O…

A: THE P-VALUE'S DEFINITION Given that the null hypothesis is true, the p-value is the likelihood of…

Q: You are doing a blood typing test and you have observed no clumping in both anti-A serum and anti-B…

A: If no clumping is seen, the blood type is O

Q: Why monoclonal antibodies method is better than polyclonal antibodies method in detecting specific…

A: Answer: Introduction: Monoclonal antibodies (mAbs) are formed by presenting an antigen to a mouse…

Q: Ifan individual was immunized tirst with Pathogen X, and then later with Pathogen X and Y, which of…

A: The immune system refers to the ability of the body to defend against the pathogen. The pathogen…

Q: What about a Katz Test? How is this different or similar to Barthel’s Test?

A: Katz activities of daily living (ADL) test is a gold-standard test used to quantify the functional…

Q: Outline the principle behind the following techniques:…

A: Immunofixation is an immunological technique that involves antigens and antibodies. Southern blot is…

Q: Why does the eliza with two antibodies incubate after adding the substrates?

A: ELISA are a type of immunoassay that are commonly used to quantify the specific target within a…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.