Outline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.
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Outline the principle behind the following techniques:
1. Immunofixation
2. Southern blotting
3. Ion exchange chromatograph
4. SDS-PAGE.
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- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..In your own words, explain why this procedure is referred to as an enzyme-linked immunosorbent assay.
- Which of the following represents the correct order of experimental steps for the Western Blot? A) SDS-PAGE -> Immunoblotting -> Transfer -> Detection B) SDS-PAGE -> Transfer -> Immunoblotting -> Detection C) Immunoblotting -> Transfer -> SDS-PAGE -> Detection D) Immunoblotting -> Detection -> Transfer -> SDS-PAGEWhat are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast to an indirect immunoblotting method.Explain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity and robustness when compared to other test methods? Please discuss refering to original articles as examples Covid 19.
- Describe 2 methods of immunotherapy currently in use, and the clinical results of one of these (ie, how often does it work well?)write down the following techniques of immunology and thier applications; 1.precipitation 2.agglutination 3. simple immunodiffusion 4. double immunodiffusion 5.immunoelectrophoresis 6. two dimensial and counter immnuoelectrophoresis 7.immunofluorescence 8.complement fixationWhat are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?