II. ATP ACCOUNTING, Provide what is being asked for. Show all relevant calculations and summarize your answers in the table below. A. Consider the following types of cell and their respective conditions: iv. a yeast cell undergoing fermentation but with defective alcohol dehydrogenase (hint: In yeast, alcohol dehydrogenase is responsible for shuttling reducing equivalents of cytosolic NADH to the mitochondria.)* In these cells/tissues, determine the following from the catabolism of the 2.5 moles of the disaccharide lactose (will be hydrolyzed first to yield glucose and galactose). a. Net ATP from glycolysis b. ATP from oxidative decarboxylation (if applicable) C. ATP formed from Krebs cycle (if applicable) d. Total net ATP Summarize your final answers in the given table and append your complete solutions in the following page. Write zero (0) in the box if it is your answer. B. LACTOSE a b C d IV. a yeast cell undergoing fermentation but with defective alcohol dehydrogenase (hint: In yeast, alcohol dehydrogenase is responsible for shuttling reducing equivalents of cytosolic NADH to the mitochondria.)* Assume that the deficiency is isolated and will not influence the function of other respiration components
Electron Transport Chain
The electron transport chain, also known as the electron transport system, is a group of proteins that transfer electrons through a membrane within mitochondria to create a gradient of protons that drives adenosine triphosphate (ATP)synthesis. The cell uses ATP as an energy source for metabolic processes and cellular functions. ETC involves series of reactions that convert redox energy from NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H)) and FADH2(flavin adenine dinucleotide (FAD)) oxidation into proton-motive force(PMF), which is then used to synthesize ATP through conformational changes in the ATP synthase complex, a process known as oxidative phosphorylation.
Metabolism
Picture a campfire. It keeps the body warm on a cold night and provides light. To ensure that the fire keeps burning, fuel needs to be added(pieces of wood in this case). When a small piece is added, the fire burns bright for a bit and then dies down unless more wood is added. But, if too many pieces are placed at a time, the fire escalates and burns for a longer time, without actually burning away all the pieces that have been added. Many of them, especially the larger chunks or damp pieces, remain unburnt.
Cellular Respiration
Cellular respiration is the cellular process involved in the generation of adenosine triphosphate (ATP) molecules from the organic nutritional source obtained from the diet. It is a universal process observed in all types of life forms. The glucose (chemical formula C6H12O6) molecules are the preferred raw material for cell respiration as it possesses a simple structure and is highly efficient in nature.
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