Substrate, uM VO, uM/min 2.5 28 4 40 10 70 20 95 40 112 100 128 200 139 1000 140

Complete Textbook Of Phlebotomy
5th Edition
ISBN:9781337464314
Author:Hoeltke
Publisher:Hoeltke
Chapter5: Phlebotomy Equipment
Section: Chapter Questions
Problem 1.2E
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Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed at 100 nM.

 

Using a Lineweaver-Burke plot, calculate the Km value for this reaction. Report your answer to three significant figures in units of uM.

Substrate, uM
VO, uM/min
2.5
28
4
40
10
70
20
95
40
112
100
128
200
139
1000
140
Transcribed Image Text:Substrate, uM VO, uM/min 2.5 28 4 40 10 70 20 95 40 112 100 128 200 139 1000 140
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