(iii) Explain why DNA in the presence of monovalent cations will have a high melting temperature (Tm). (iv) Erwin Chargaff's research was based on the selection of DNAs from many organisms (Table 1). If Erwin's analyses is based on only the data of Escherichia coli (K12), would he be able to establish certain Chargaff's rule? Explain.
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- In a series of infection experiments, a researcher discovers that the ID50 value for the infectious bacterium Parasiticum mucoides is 2,000, and that the ID50 for another infectious bacterium, Donoteatum thisbacterium, is 150. Given these data, a person exposed to 1,000 bacteria of each type would be more likely to be infected by which bacterium? There is no way to know given the information provided Both infections are equally likely Donoteatum thisbacterium Parasiticum mucoidesWhat is the molecular weight of the Botulinum neurotoxin, a protein that contains 1350 amino acids? Assume an average distribution of amino acidsWhy is it not safe to assume that two microorganisms with the same G + C content belong to the same species? In what ways are G + C content data taxonomically valuable?
- Erwin Chargaff did not have any DNA samples from thermoacidophilic bacteria such as those that thrive in the geothermal (hot) springs of Yellowstone Park. Such bacteria had not been isolated in 1951 when Chargaff reported his results. If he had obtained such a sample, what do you think its relative G-C content might have been? Why?Succinic acid is produced by Actinobacillus succinogenes with the following medium composition (g/L): glucose, 50; yeast extract, 5; NaCl, l; Na2HPO4, 0.3; NaH2PO4, 1.4; K2HPO4, 1.5; MgC12.6H20, 0.2; CaC12.2H20, 0.23. The pH is controlled and maintained at 3.63. Estimate the range of upper limit, if 0.8% of deadband is applied. With a schematic diagram, explain how to maintain the pH throughout the fermentation process.. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…
- Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? a. Isoelectric focusing b. Hydrophobic chromatography c. Affinity chromatography d. DialysisExplain.The rate of migration of nucleic acid fragments through an agarose gel is determined by the_Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
- If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the concentration of original protein?Complete the table below by adding (+) or (-) if eahc sample (amino acid or protein) below will test positive or negative, respectively. sample BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOSKIN-COLE G W H Y C R HAIR GELATIN CASEIN PEPTONE Identify the amino acid that will give the following data: BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOPSKIN-COLE - + + + - - + - Name of amino acid: structurte of the amino acid:How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution