In a study of several characters, it was found that when di-hybrid crosses were made, several genes did not show independent behavior, so it was decided to develop the chromosomal map of five genes, and to know their position within the chromosome. According to the test crosses, the following results were obtained. a'b*d* 1250 b*d* e* 980 d' e* f* 835 ab d a* b d def d'e f 1235 bde 985 840 645 b*d e 4 195 a b* d* 640 b d*e* 2 d e'f* 200 a' b'd 320 b*d* e 320 d'e*f 3 ab d* 330 bd e* 325 de f 1 a* b d* 3 b* de* 250 d' e f* 410 a b+ d 2 b d'e 255 d e' f 405 Mapping by determining the position and distance of genes within the chromosome

In a study of several characters, it was found that when di-hybrid crosses were made, several genes did not show independent behavior, so it was decided to develop the chromosomal map of five genes, and to know their position within the chromosome. According to the test crosses, the following results were obtained. a'bd 1250 bd e* 980 d' e* f* 835 ab d a* b d def d'e f 1235 bde 985 840 645 bd e 4 195 a b d* 640 b de 2 d e'f* 200 a' b'd 320 bd e 320 d'ef 3 ab d 330 bd e* 325 de f 1 a* b d* 3 b* de* 250 d' e f* 410 a b+ d 2 b d'e 255 d e' f 405 Mapping by determining the position and distance of genes within the chromosome

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Description: In a study of several characters, it was found that when di-hybrid crosses were made,several genes did not show independent behavior, so it was decided to develop thechromosomal map of five genes, and to know their position within the chromosome.Acc

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter8: Patterns Of Inheritance

Section: Chapter Questions

Problem 2ACQ: Figure 8.10 In pea plants, purple flowers (P) are dominant to white (p), and yellow peas (Y) are...

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Figure 8.10 In pea plants, purple flowers (P) are dominant to white (p), and yellow peas (Y) are dominant to green (y). What are the possible genotypes and phenotypes for a cross between PpYY and ppYy pea plants? How many squares would you need to complete a Punnett square analysis of this cross?
In a diploid plant species, an F1 with the genotype Mm Rr Ss is test crossed to a pure breeding recessive plant with the genotype mm rr ss. The offspring genotypes are as follows: Genotype Number Mm Rr Ss 687 Mm Rr ss 5 Mm rr Ss 68 Mm rr ss 196 mm Rr Ss 185 mm Rr ss 72 mm rr Ss 8 mm rr ss 679 Total 1900 1. Why is the recombination frequency for the outside pair of genes not equal to the sum of the recombination between the adjacent gene pairs?
In a series of two-point map crosses involving five genes locatedon chromosome II in Drosophila, the following recombinant (single- crossover) frequencies were observed:pr–adp 29pr–vg 13pr–c 21pr–b 6adp–b 35adp–c 8adp–vg 16vg–b 19vg–c 8c–b 27 In another set of experiments, a sixth gene (d) was testedagainst b and pr, and the results were d - b = 17% andd - pr = 23%. Predict the results of two-point mapsbetween d and c, d and vg, and d and adp.
Homozygous wild-type male mice (AA BB CC) were crossed with triplemutant female mice (aa bb cc), forming an F1 generation with the followinggenotype (Aa Bb Cc). The F1 males were crossed with triple mutantfemales, forming the following F2 phenotypes”“a B c” 3“A b C” 3“a b c” 8“A B c” 5“a b C” 5“A B C” 8“a B C” 6“A b c” 6 44 Determine the sequence of the genes
In a diploid plant species, an F1 with the genotype Mm Rr Ss is test crossed to a pure breeding recessive plant with the genotype mm rr ss. The offspring genotypes are as follows: Genotype Number Mm Rr Ss 687 Mm Rr ss 5 Mm rr Ss 68 Mm rr ss 196 mm Rr Ss 185 mm Rr ss 72 mm rr Ss 8 mm rr ss 679 Total 1900 1. What is the gene order of these linked genes?
in a cross AaBb X aabb, the following progeny were obtained: AaBb=50 aabb=46 Aabb=7 aaBb=6 What is the recombination frequency between the genes A and B? How far apart are they if they were on the same chromosome
In a diploid plant species, an F1 with the genotype Mm Rr Ss is test crossed to a pure breeding recessive plant with the genotype mm rr ss. The offspring genotypes are as follows: Genotype Number Mm Rr Ss 687 Mm Rr ss 5 Mm rr Ss 68 Mm rr ss 196 mm Rr Ss 185 mm Rr ss 72 mm rr Ss 8 mm rr ss 679 Total 1900 1. Calculate the recombination frequency between each pair of genes
An individual heterozygous for four genes, A/a • B/b •C/c • D/d, is testcrossed with a/a • b/b • c/c • d/d, and 1000progeny are classified by the gametic contribution ofthe heterozygous parent as follows:a • B • C • D 42A • b • c • d 43A • B • C • d 140a • b • c • D 145a • B • c • D 6A • b • C • d 9A • B • c • d 305a • b • C • D 310a. Which genes are linked?b. If two pure-breeding lines had been crossed toproduce the heterozygous individual, what would theirgenotypes have been?c. Draw a linkage map of the linked genes, showing theorder and the distances in map units.d. Calculate an interference value, if appropriate
From a series of two-point crosses, the following mapdistances were obtained for the syntenic genes A, B,C, and D in peas:B ↔ C 23 m.u.A ↔ C 15 m.u.C ↔ D 14 m.u.A ↔ B 12 m.u.B ↔ D 11 m.u.A ↔ D 1 m.u.Chi-square analysis cannot reject the null hypothesis of no linkage for gene E with any of theother four genes.a. Draw a cross scheme that would allow you todetermine the B ↔ C map distance.b. Diagram the best genetic map that can be assembled from this data set.c. Explain any inconsistencies or unknown features inyour map.d. What additional experiments would allow you toresolve these inconsistencies or ambiguities?
three recessive genes a, b, and c in the model plant Arabidopsis are found to be linked on chromosome 4. A three point test cross is done with a homozygous recessive plant with a heterozygous for all three genes. Following is the number of progenies a b C 65 A B c 56 A B C 1267 a b c 1310 A b C 550 a B c 515 a B C 470 A b c 489 Total = 4,722 Determine the middle locus by your choice of method and after that calculate the map distance between the genes in map unit (m.u.).
In a series of two-point map crosses involving five genes locatedon chromosome II in Drosophila, the following recombinant (single-crossover) frequencies were observed:pr–adp 29pr–vg 13pr–c 21pr–b 6adp–b 35adp–c 8adp–vg 16vg–b 19vg–c 8c–b 27 If the adp gene is present near the end of chromosome II(locus 83), construct a map of these genes.
To determine the recombination frequency between body color and wing genes in flies, you perform several crosses where you cross an F1 having red body and smooth wings with a yellow-bodied, crinkle-winged fly. You get the following results. What is the distance between the genes for body color and wing surface in map units?