In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of the haploid fungus Neurospora crassa (n = 7) moves slowly but eventually forms seven bands, which represent DNA fractions that are of different sizes and hence have moved at different speeds. These bands are presumed to be the seven chromosomes. How would you show which band corresponds to which chromosome?

In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of the haploid fungus Neurospora crassa (n = 7) moves slowly but eventually forms seven bands, which represent DNA fractions that are of different sizes and hence have moved at different speeds. These bands are presumed to be the seven chromosomes. How would you show which band corresponds to which chromosome?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter6: Cytogenetics: Karyotypes And Chromosome Aberrations

Section: Chapter Questions

Problem 1QP: Analyzing Karyotypes 1. Originally, karyotypic analysis relied only on size and centromere placement...

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In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of thehaploid fungus Neurospora crassa (n = 7) moves slowlybut eventually forms seven bands, which represent DNAfractions that are of different sizes and hence havemoved at different speeds. These bands are presumed tobe the seven chromosomes. How would you show whichband corresponds to which chromosome?
Colchicine is a chemical mutagen that inhibits the spindle formation and prevents anaphase, which retains the cell’s single restitution nucleus (doubled chromosome number). Suppose that an onion (2n=16) is subjected to three consecutive rounds of colchicine treatment, what will be the resulting chromosome number of the treated onion?
A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box). Solve, (a) Since everyone has two copies of each chromosome and therefore, two alleles of each gene, what accounts for the appearance ofonly one allele at some loci? (b) Which suspect is a possible source of the blood? (c) Could the suspect be identifi ed using just one of the three STR loci? (d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?
Here are the results of the testcross:Green-eyes, wild-type wings 245Green-eyes, miniature wings 232Wild-type eyes, wild-type wings 250Wild-type eyes, miniature wings 2386.) In your opinion, is the grn gene on the same chromosome as the min gene? How do you know?Next, you set up another experiment to determine if grn is on chromosome #1. The only appropriate fliesthat you have handy are double-mutants for the black-body mutation (blk) and curved wings mutation(crv), but this should work just fine. First, you conduct a series of crosses to generate a strain of triple-mutant flies (grn blk crv). Then, you cross those with wild-type flies to generate triple hybrids.7.) What are the genotype(s) and phenotype(s) of the progeny of this cross?Next, you set up a testcross to reveal any linkage between the three genes.8.) What are the genotypes and phenotypes of this testcross?
A heterozygous reciprocal translocation involving 2 non-homologous chromosomes resulted to a new sequence ABCDXYZ and TUVWEFG. Illustrate the chromosomes before and after translocation and the pachytene configuration of the chromosome after translocation.
If you are comparing the two telomeres in each entryin the following list, in which cases would you expectthe two telomeres always to have exactly the samenumber of TTAGGG repeats?a. One telomere at one end of a chromosome, one telomere at one end of a nonhomologous chromosome.b. One telomere at one end of a chromosome, onetelomere at the corresponding end of the homologous chromosome.c. One telomere at one end of a chromosome, theother telomere at the other end of the samechromosome.d. One telomere at one end of a chromatid, the othertelomere at the corresponding position in the sisterchromatid.
The a, b, and c loci are all on different chromosomesin yeast. When a b+ yeast were crossed to a+ b yeastand the resultant tetrads analyzed, it was found thatthe number of nonparental ditype tetrads was equal tothe number of parental ditypes, but there were no tetratype asci at all. On the other hand, many tetratypeasci were seen in the tetrads formed after a c+ wascrossed with a+ c, and after b c+ was crossed withb+ c. Explain these results.
Saccharomyces cerevisiae is a ____Budding yeast have two mating types: _______.-cells. The expression of________. observed in this experiment after a _______. cross was made. Diploid cells contain_________. of the number of chromosomes found in haploid cells. The number of yeast haploid strains used in this experiment to make the dihybrid cross is _________ When a diploid cell undergoes mitosis, the number of chromosomes in the resulting cells ________ When a diploid cell undergoes meiosis, the number of chromosomes in the resulting cells are __________ The result of __________. is the formation of gametes. Alleles are alternate forms of a __________ A heterozygous organism for a specific trait will express the phenotype determined by the _________. allele. If two alleles separate during meiosis and gamete formation, then this is an example of Mendel’s Law of _________ In yeast, the alleles for colony colour and tryptophan dependence are located on different chromosomes.…
Map distances were determined for four differentgenes (MAT, HIS4, THR4, and LEU2) on chromosome III of the yeast Saccharomyces cerevisiae:HIS4 ↔ MAT 37 cMTHR4 ↔ LEU2 35 cMLEU2 ↔ HIS4 23 cMMAT ↔ LEU2 16 cMMAT ↔ THR4 20 cMWhat is the order of genes on the chromosome?
If you are comparing the two telomeres in each entryin the following list, in which cases would you expectthe two telomeres always to have exactly the samenumber of TTAGGG repeats?a. One telomere at one end of a chromosome, one telomere at one end of a nonhomologous chromosome.b. One telomere at one end of a chromosome, onetelomere at the corresponding end of the homologous chromosome.
Two strains of S. cerevisae (yeast) are crossed. One has the genotype A B and the other a b. Which statement correctly describes the tetrads that can be produced by one reciprocal exchange between the A and B genes without gene conversion in the dihybrid? A) Recombination occurs at the four-strand stage to produce a tetrad with A B, A B, a B, and a b chromosomes. B) Recombination occurs at the four-strand stage to produce a tetrad with A B, A b, a B, and a b chromosomes. C) Recombination occurs at the two-strand stage to produce a tetrad with A B, A b, a B, and a b chromosomes. D) Recombination occurs at the two-strand stage to produce a tetrad with A b, A b, a B, and a B chromosomes.
You are investigating two temperature-sensitive mutant cells. In one, you can inactivate shugoshin activity by raising the temperature slightly at the start of meiosis. In the other, you can similarly inactivate separase activity by raising the temperature. How would the two cells be affected by inactivating these proteins?