In John Gurdon's nuclear-transfer experiments, he used nuclei from tadpole intestinal cells. Do you think he would have had more success or less success if he had taken cells from a blastula? From an adult frog? Please briefly justify your answers
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- In John Gurdon's nuclear-transfer experiments, he used nuclei from tadpole intestinal cells. Do you think he would have had more success or less success if he had taken cells from a blastula? From an adult frog? Please briefly justify your answersYou got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions: a) Which technique will you use to isolate mitochondria? b) What happens to the pH of the medium when the medium is kept anaerobic? c) What happens when O2-saturated saline is added to the mixture?The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells (CER) cells. The infection was monitored by looking for specific viral inclusions using anti-rabies fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various lengths of time after virus attachment with ammonium chloride and chloroquine. The results are show in Figure 2. 100 75 50 25 1 3 5 7. 24 Time (h) (1) 20 mM NHẠCI (0) 0.1 mM chloroquine Figure 2 What is the effect of ammonium chloride on the infection of CER cells? Explain why there are such effects. Fluorescence inhibition (%)
- Please answer substantially, explain it in your own words, don't copy and paste in any website. 1. Explain why a lysosome remains unaffected even if it stores powerful digestive enzymes known as lysozymes. 3. How do microscopes differ in their functions and capabilites in appreciating minute life forms?short description about the specimen of humam cheek cells (animal cells)Question 2. Why are viruses notconsidered to be organisms and notdescribed as being alive?1) Virions are unable to reproduce by themselves.2) Virions are not able to metabolize by themselves.3) Virions are not able to synthesize DNA by themselves.4) Virions are not able to assemble spontaneously.A.1B.2C. 3D. 4E. 1, 2, and 3
- Three actively growing cultures of the same strain of yeast (saccharomyces cerevisiae) are used. Culture A has been incubated for 48 hours and Culture B has been incubated for 24hours and Culture C for 6 hours. a) Make a drawing of the cells from each culture to identify the various stages of the cell division. b) Is there any difference between the 3 cultures?I am more so wondering about the structural differences between the two cells, like the organelle arrangment and receptors.The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells (CER) cells. The infection was monitored by looking for specific viral inclusions using anti- rabies fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various lengths of time after virus attachment with ammonium chloride and chloroquine. The results are show in Figure 2. 100 75 s0 I 3 5 7 Time (h) 24 (m) 20 mM NH,CI (0) 0.1 mM chloroquine Figure 2 What is the effect of treatment of CER cells with chloroquine? Explain. Fluorescence inhibition (%)
- Why was a donor cell extracted from champion horseWhy must primary cell cultures be restarted every so often when preparing primary cell cultures to observe morphological changes caused by cells infected by a virus? Why are tumor cells preferred? Why are non-enveloped viruses generally more resistant to disinfectants than are enveloped viruses? A public health physician isolated large number of phages from rivers used as a source of drinking water in western Africa. They physician is very concerned that humans might become ill from drinking this water, although she knows that the phages specifically attack bacteria. Why is she concerned?One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/