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Name: In most experiments using the lac operon, researchers use the syntheti
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Description: In most experiments using the lac operon, researchers use the synthetic inducer IPTG to turn on operon expression, instead of lactose or allolactose. What do you think is the advantage of using IPTG?

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Biology

In most experiments using the lac operon, researchers use the synthetic inducer IPTG to turn on operon expression, instead of lactose or allolactose. What do you think is the advantage of using IPTG?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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Similar questions

A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
The lac operon produces enzymes that allow the bacteria E. coli to metabolize lactose, it is in an inducable operon. When lactose is present, will the repressor protein attach or leave the operon? How would this effect the production of lactose producing enzymes?
What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)
If you make a large deletion in the lac operon to remove the operator region, will the structural proteins of the lac operon be made?
Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absent
In the lac operon, the lacZ gene codes for an enzyme called ____________, which catalyze the breakdown of the sugar __________ into monosaccharides. This enzyme can also hydrolyze the sugar analog ____________, which produces blue pigments following its breakdown. Since the lac operon is not always on the bacterial cells, we add the chemical ________ which prevents the lac depressor from binding to the operators, leading to constitutive expression of the operon.
Why is the TRP operon considered a repressible operon, while the LAC operon is considered an inducible operon?
Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation may block the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operon in a way that prevents the repressor protein from binding to the operator. How would you distinguish between these two possibilities?
If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not alter the conformation of the repressor protein, how would the expression of the lac operon be affected?
What is the difference between positive regulation of the lac operon and negative regulation of the lac operon?