Biology (MindTap Course List)
Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Textbook Question
Chapter 14, Problem 9TYU

A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription

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If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?   a)  There is a mutation in the repressor protein   b)  There is a mutation in the catabolite activator protein   c)  There is a mutation in the promoter region   d)  There is a mutation in the luxI protein
1.  a) If glucose is not available and lactose is absent from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?  b)If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?             b)
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  • Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation mayblock the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operator in a way that prevents lac repressor from binding to the operator. How would you distinguish between these two possibilities?
    How would each manipulation of the leader region of the trp mRNA affect transcription of the E. coli trp operon?   1. Increasing the distance (number of bases) between the leader peptide gene and sequence 2 would ___________________ transcriptional attenuation of the trp operon. (increase/decrease/not change) 2. Increasing the distance between sequences 2 and 3 would ____________________ transcriptional attenuation of the trp operon. (increase/decrease/not change) 3. Removing sequence 4 would ________________ transcriptional attenuation of the trp operon. (cause/not change/prevent) 4. Changing the two Trp codons in the leader peptide gene to His codons would cause transcriptional attenuation of the trp operon to ____________________. (lose its sensitivity to histidine/increase its sensitivity to tryptophan/lose its sensitivity to tryptophan) 5. Eliminating the ribosome‑binding site for the gene that encodes the leader peptide would cause transcriptional attenuation of the trp operon to…
    If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a inducible, constitutive or repressible operon?
  • Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation may block the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operon in a way that prevents the repressor protein from binding to the operator. How would you distinguish between these two possibilities?
    Operons involved with the biosynthesis of molecules such as amino acids are most likely to be regulated in which of the following ways? a. The product of the biosynthetic pathway represses transcription. b. The product of the biosynthetic pathway activates transcription. c. A precursor of the biosynthetic pathway represses transcription. d. A precursor of the biosynthetic pathway activates transcription.
    Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented.   Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent.                                    Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow                        Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
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