Chamistry In my biochemistry course, we arecompleting a project where we take thehuman insulin degrading enzyme (IDE) andmake a mutation, propose what wouldhappen upon said mutation, and determinemethods for testing this in the lab. Themutation I chose is at the K571C residue,lysine 571 in domain C. Since lysine ispositively charged at a neutral pH, I amchoosing to replace it with serine, whichhas a neutral charge. Lysine 571 is also partof a salt bridge between Aspartic Acid 426,D426C. By replacing lysine with serine, Ihypothesize that the salt bridge will bebroken and will then increase the catalyticactivity of the enzyme.Based off of this background, what wouldbe some useful methods that could be usedto test this in the lab?

Insulin-degrading enzyme (IDE) is zinc-metalloendopeptidase that hydrolyzes insulin and other…

Answered: In my biochemistry course, we are…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter4: Cells

Section: Chapter Questions

Problem 1ITD

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In my biochemistry course, we are completing a project where we take the human insulin degrading enzyme (IDE) and make a mutation, propose what would happen upon said mutation, and determine methods for testing this in the lab. The