In part 2 you ran your samples on an agarose gel. Explain how agarose gel electrophoresis works including visualisation of DNA, and why a DNA ladder was needed
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In part 2 you ran your samples on an agarose gel. Explain how agarose gel electrophoresis works including visualisation of DNA, and why a DNA ladder was needed
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Solved in 2 steps
- Use a drawing to illustrate the principle of DNA gel electrophoresis. (2 marks)-+You set aside some of your purified PCR product to run on a gel. Name two things we learn by running our DNA on an agarose gel electrophoresis?The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
- Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either pole but in fact moves by diffusion through the TAE buffer in the agarose. b. DNA can be attracted to either pole depending on the bases it is made of. c. DNA is attracted to the negative pole. d. DNA is attracted to the postive poleWhich of the following correctly describes a possible scenario during a run of gel electrophoresis with DNA samples?
- Quantitative PCR (qPCR) has one extra ingredient to quantify DNA. Describe its structure and explain how it changes during the reaction.What is the purpose of Bromophenol Blue in electrophoresis? a. To follow the progress of the gel as it is running b. It acts as a DNA size ladder or DNA size standards. c. To allow the electric current to move through the gel. d. To visualize the DNA after the gel has finished running. What is the purpose of acetate in TAE? a. It helps to maintain the pH of the buffer b. To carry electrons to allow the electric current to travel through the gel. c. It is used to stop the action of DNases d. It is required to see the DNA on a UV light.This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.
- Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode? A. DNA is positively charged to attracted to the negative end of the unit B. the agarose gel is positively charged C. the agarose gel in negatively charged D. DNA is negatively charged so attracted to the positive end of the unitIn 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.Which of the following statements about gel electrophoresis is false? Choose all that apply. Smaller DNA fragments travel faster and further during gel electrophoresis. DNA fragments can be separated based on fragment length. DNA fragments can be separated based on DNA sequence. Larger DNA fragments travel faster and further during gel electrophoresis.
