In polarized MDCK cells, proteins X and Y normally are located to the apical plasma membrane domain, and are not observed to any significant degree in the basolateral membranes of these cells. To investigate the manner in which these two proteins arrive at this subcellular localization you transform these cells with a plasmid containing a dominant-negative form of one of the proteins of the AP-2 adaptor protein complex and observe the subsequent distribution of proteins X and Y. After treatment, protein X remains at the apical membrane, but protein Y now is primarily observed in the basolateral membranes. Explain how expression of a dominant-negative AP-2 complex can affect the subcellular distribution of protein Y but not protein X?
In polarized MDCK cells, proteins X and Y normally are located to the apical plasma membrane domain, and are not observed to any significant degree in the basolateral membranes of these cells. To investigate the manner in which these two proteins arrive at this subcellular localization you transform these cells with a plasmid containing a dominant-negative form of one of the proteins of the AP-2 adaptor protein complex and observe the subsequent distribution of proteins X and Y. After treatment, protein X remains at the apical membrane, but protein Y now is primarily observed in the basolateral membranes. Explain how expression of a dominant-negative AP-2 complex can affect the subcellular distribution of protein Y but not protein X?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In polarized MDCK cells, proteins X and Y normally are located to the apical plasma membrane domain, and are not observed to any significant degree in the basolateral membranes of these cells. To investigate the manner in which these two proteins arrive at this subcellular localization you transform these cells with a plasmid containing a dominant-negative form of one of the proteins of the AP-2 adaptor protein complex and observe the subsequent distribution of proteins X and Y. After treatment, protein X remains at the apical membrane, but protein Y now is primarily observed in the basolateral membranes. Explain how expression of a dominant-negative AP-2 complex can affect the subcellular distribution of protein Y but not protein X?
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