In the lac operon of E. coli, if glucose levels increase while lactose levels decrease, what will happen in the regulation of genes controlling the enzymes needed to catabolize lactose? the lac operon will be activated due to cAMP-assisted CAP binding to a site near the promoter the lac operon will be activated due to CAP detachment from a site near the promoter the lac operon will be repressed due to cAMP-assisted CAP binding to a site near the promoter the lac operon will be repressed due to CAP detachment from a site near the promoter all of the above

In the lac operon of E. coli, if glucose levels increase while lactose levels decrease, what will happen in the regulation of genes controlling the enzymes needed to catabolize lactose? the lac operon will be activated due to cAMP-assisted CAP binding to a site near the promoter the lac operon will be activated due to CAP detachment from a site near the promoter the lac operon will be repressed due to cAMP-assisted CAP binding to a site near the promoter the lac operon will be repressed due to CAP detachment from a site near the promoter all of the above

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
Mutations may have an effect on the expression of the lac operon and the trp operon. Would the following mutations have a cis- or transeffect on the expression of the protein-encoding genes in the operon? A. A mutation in the operator site that prevents lac repressor from binding to it B. A mutation in the lacI gene that prevents lac repressor from binding to DNA C. A mutation in the trpL gene that prevents attenuation
A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose. What will be the effect of this mutation in the presence of allolactose (assume glucose is absent)? a) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon is blockedb) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon is blocked c) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon increasesd) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon increases
An E. coli cell acquires a mutation in the gene that encodes CAP. The mutation affects the DNA-binding domain of CAP and ablates the ability of CAP to bind to the CAP site. Under which condition will the genes of the lac operon be transcribed at high levels? Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose absent Glucose present, lactose present Under any of these conditions, the lac operon will not be transcribed at high levels
1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production
Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absent
In a bacterial cell with the genotype I-: P+: O+: Z+: Y+: A+, which of the following expression patterns of the lac operon would you see? (All + signs designate functional units and - signs designate non-functional units) A. No expression of Z, Y and A under any environmental conditions. B. Z, Y and A would always be expressed. C. Z, Y and A would be expressed when lactose was present but not expressed if lactose is not present. D. Z, Y and A would be expressed in the absence of lactose, but not when lactose was present. E. None of the above.
If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is present
Which of the following condition(s) is optimal for expression of the lac operon? I. low glucose and formation of CAP-cAMP complex II. high glucose resulting in no cAMP III. no lactose present and a functional repressor IV. presence of lactose binding to a functional repressor
Consider the trp operon found in E. coli. You have discovered a mutant strain of E.coli that contains a mutation in its trpR gene. This mutation prevents the trpR protein from binding to its small effector molecule. What would happen if both the wild type and mutant bacteria were put in an environment that contained lots of tryptophan? The normal (wild type) bacteria would have (i)________expression of the trp operon while the mutant bacteria would have (ii) _________ expression of the trp operon. A) (i) high (ii) high B) (i) low (ii) low C) (i) high (ii) low D) (i) low (ii) high
consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system. The fec operon promoter, called “Pfec”, is normally off when no regulators are bound to it (like the lac operon promoter). The activator FecA senses iron citrate and brings the appropriate enzyme to Pfec to start fec transcription when iron citrate levels are high. The repressor FecB senses free iron atoms and prevents fec transcription when free iron levels are high by binding to an operator between the promoter and structural genes. Considering the fecoperon described above, under which conditions would the operon be off? Choose all that apply. Low levels of free iron and high levels of citrate High levels of free iron and low levels of citrate High levels of free iron and high levels of citrate Low levels of free iron and low levels of citrate