For the lac operon, when glucose is absent and lactose is present ___________ None of the options cAMP-CAP is bound to the operator and the repressor is not bound to the promoter cAMP-CAP is not bound to the promoter and the repressor is bound to the operator cAMP-CAP is bound to the promoter and the repressor is not bound to the operator
Q: A mutation at the operator prevents the regulator protein from binding. What effect will this…
A: An operon includes a group of genes that have a common promoter, regulator, and would be transcribes…
Q: Maltose utilization in E. coli requires the proteins encoded by genes in three different operons.…
A: It is given that the maltose utilisation in E.coli requires the proteins which are encoded by genes…
Q: In the absence of lactose, the genes of the lac operon are: Not transcribed; because there is…
A: Introduction In E. coli and numerous other enteric bacteria, the lactose operon (lac operon) is a…
Q: Which of the following statements can be made concerning the role of the lac repressor (Lac R) in…
A: The lac repressor is a protein that binds to the promoter sequence of the lac operon and inhibits…
Q: ttenuator control of the Trp operon what is the correct option from the choices below? involves a…
A: The trp operon, found in E. coli bacteria, is a group of genes that encode biosynthetic enzymes for…
Q: Regulation of the E. coli trp operon by attenuation requires the trp repressor protein. prevents…
A: An operon is a function unit of DNA containing a set of genes including structural gene, a…
Q: In the lac operon, how would gene expression be affected if each one of the following segments was…
A: The lac operon is basically a group of genes with a single promoter whose genes encode proteins that…
Q: Components necessary for regulating the /trp/ operon do NOT include
A: Answer - Option E - CAP protein
Q: In order for the lac operon to be triggered, lactose must be present and ________. a. cAMP levels…
A:
Q: I. CAP-CAMP complex binds to the operator locus blocking the RNA polymerase. II. In the presence of…
A: The lactose operon (also known as the lac operon) is a set of genes that are specific for uptake and…
Q: A mutation in the lac I gene that prevents production of the lac repressor choose an answer below:…
A: lac operon also known as lactose operon was discovered by F. Jacob and J. Monod, for that, they…
Q: Match the correct answer for when an E. coli cell with a functional lac operon is growing in the…
A: When glucose and lactose are present in medium then E.coli uses glucose first and inhibits the…
Q: A novel operon is controlled by two regulatory systems that function in a hierarchy. The first is…
A: An operon can be defined as the cluster of coordinately controlled and regulated genes. It is…
Q: Which of the below conditions would result in the lowest level of expression from the lac operon…
A: Option C is correct :
Q: In E. coli, when glucose is present, galactose is used to make a component of the bacterial cell…
A: In the absence of glucose the operon is not described or transcribed at very low levels. When…
Q: In E. coli, when glucose is present, galactose is used to make a component of the bacterial cell…
A: As statement states that the galactose used for making the component of bacterial cell wall in the…
Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…
A: Tryptophan operon found in E.coli and some other bacteria. It is group of genes which encodes for…
Q: he lac operon, determine whether genes will be expressed (on) or silenced (off) given the following:…
A: IN option A Lactose levels are high, glucose is low.---- Genes will be expressed(on).
Q: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the…
A: Answers - 1) False 2) True 3) True There are 3 structural genes in lac operon - lac Z, lac Y and…
Q: You mutated the gene encoding the cyclic AMP receptor protein (CRP). In this mutant, the CRP is…
A: Operon is a gene regulatory structure that contains clusters of genes and these clusters of genes…
Q: You have cells that are mutant in CAP protein such that it cannot bind to CAMP. For these mutant…
A: Cap protein- In solution, the catabolite activator protein (CAP, also known as cAMP receptor…
Q: Maltose utilization in E. coli requires the proteins encoded by genes in three different operons.…
A: The process of maltose utilization in E.coli requires the proteins encoded by genes in three…
Q: High levels of glucose in the environment result in low levels of transcription of the lac operon.…
A: Answer- glucose lowers the levels of cAMP, which is necessary to activate the CAP protein
Q: The lac operon has which of the following characteristics? O 1) usually requires an activator…
A: In Eukaryotes all genes are separate and produce individual mRNAs on transcription. However, in…
Q: When would the lac operon in E. coli be repressed, and why?
A: Operon includes a group of structural genes and regulatory sequences.
Q: In the lac operon system, lactose is considered the ___________, and when the gene lacI is mutated…
A: The genes are the hereditary unit of an organism which are passed on from the parental generation to…
Q: For each of the following conditions in the Lac Operon, state whether transcription will occur or…
A: The lac operon of E. coli contains genes responsible in lactose metabolism. It is expressed only…
Q: consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system.…
A:
Q: Assuming that the level of glucose is low, a mutation in the repressor associated with the lac…
A: The lac operon which is also known as lactose operon is a set of genes that are specific for uptake…
Q: For each description, state if the gene expression will increase (1), decrease (2) or remain the…
A: The transcription is a process through which information in the DNA molecule is transcribed into…
Q: Regarding the trp operon: when levels of tryptophan are low, the ___ hairpin forms, resulting in…
A: Trp operon is negatively regulated by tryptophan which means in low concentration of tryptophan the…
Q: Assume you have identified a new operon in bacteria (which you call the suc operon) tha encodes…
A: Any bacterial gene can be classified as an operon.An operon is a collection of bacterial genes with…
Q: Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the…
A: Hexose operon is the segment of DNA which includes regulatory gene, structural gene, promoter and…
Q: You have E. coli growing in HIGH lactose and HIGH glucose. You measure the amounts of protein and…
A: The E.coli is growing in a medium rich in lactose and Glucose both. There are two regulatory…
Q: The fictional bacteria Plumbumbacter aurus converts the lead ions to gold ions and uses the…
A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…
Q: Theara operon is an inducible operon that controls the production of the sugar arabinose. When…
A: An operon is a group of genes that are transcribed together and are under the influence of a single…
Q: Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the…
A: The operons are regions in DNA which have a cluster of related genes and these function as a unit.…
Q: Select all of the cis-acting regulatory elements of the lac operon. Operator CAP+cAMP CAP+cAMP…
A: lac operon: - Inducible system - Both positive and negative regulation - Involved in lactose…
Q: What are the requirements for the lac operon to be actively transcribed? a. Glucose and lactose…
A: Operons are the group of genes which are transcribed under a single promoter. These are found in…
Q: Which of the following statements about the lactose operon in E. coli is TRUE? ) It contains the…
A: An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of genes under…
Q: The tryptophan operon is a repressible operon that is A. transcribed only when glucose is present…
A: The tryptophan operon is a repressible operon that is - B. not transcribed whenever tryptophan is…
Q: A. The gene that regulates the production of the Lacl protein is found further upstream of the lac…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain…
A: Promoter is a binding site for the RNA polymerase in the gene. The RNA polymerase is involved in the…
Q: E. coli is given lactose as a nutrient source. This results in the activation of the lac operon only…
A: The solution to the above mentioned questions is as follows:
Q: When lactose is present lactose induces the activation of the genes in the lac operon by binding…
A: The Lac operon is an inducible operon that is inhibited by a repressor protein in the absence of…
Q: In the presence of arabinose sugar and absence of glucose, which of the following conditions may be…
A: Arabinose could also be a five-carbon sugar that happens naturally and can be used as a result of…
Q: consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system.…
A: The fec operon is responsible for regulating the expression of iron in the cytoplasm of cell.
- None of the options
- cAMP-CAP is bound to the operator and the repressor is not bound to the promoter
- cAMP-CAP is not bound to the promoter and the repressor is bound to the operator
- cAMP-CAP is bound to the promoter and the repressor is not bound to the operator
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- A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionINTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and activates catabolicactivator protein (CAP). b. and glucose is absent, the level of cAMP decreases. c. activated CAP binds the repressor protein to remove it from the operator gene. d. the cell prefers lactose over glucose. e. RNA polymerase cannot bind to the promoter.
- In the lac operon (above), what happens when glucose levels are low, but lactose levels increase? the repressor protein is detached from the operator, and transcription is not initiated the repressor protein is attached to the operator, and transcription is not initiated the repressor protein is detached from the operator, and transcription is initiated the repressor protein is attached to the operator, and transcription is initiated. Check all of the above occur at equal rates when glucose levels are low but lactose levels are highThe lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+
- To study the lac operon, you engineer a strain of E coli to have a lac operon in which the lac Z gene is replaced by the gene for green fluorescent protein (GFP). Expression of GFP generates a green color in the cells that can be easily quantitated with a fluorescence microscope. You test the activity of the operon in the absence of the inducer IPTG, the presence of the inducer IPTG and the presence of an antibiotic the completely inhibits RNA polymerase (i.e. no gene expression). You then use this system to test the effects of various mutation on the activity of the operon. Match the following mutations with the activity (A, B or C) you would expect to observe with the mutation. All experiments are done in the presence of IPTG unless otherwise stated.In the lac operon system, lactose is considered the ___________, and when the gene lacI is mutated such that the protein it produces cannot bind to lactose (IS mutation), the lac operon is ________. Group of answer choices repressor; never expressed activator; constitutively expressed inducer; never expressed inducer; constitutively expressed structural gene; never expressedMaltose utilization in E. coli requires the proteins encoded by genes in three different operons. One operonincludes the genes malE, malF, and malG; the secondincludes malK and lamB; and the genes in the thirdoperon are malP and malQ. The MalT protein is apositive regulator that controls the expression of allthree operons; expression of the malT gene itself iscatabolite sensitive.a. What phenotype would you expect to result from aloss-of-function mutation in the malT gene?b. Do you expect the three maltose operons to containbinding sites for CRP (cAMP receptor protein)?Why or why not?In order to infect E. coli, bacteriophage λ binds to themaltose transport protein LamB (also known as the λreceptor protein) that is found in the outer membraneof the bacterial cell. The synthesis of LamB is induced by maltose in the medium via expression of theMalT protein, as described above
- Maltose utilization in E. coli requires the proteins encoded by genes in three different operons. One operonincludes the genes malE, malF, and malG; the secondincludes malK and lamB; and the genes in the thirdoperon are malP and malQ. The MalT protein is apositive regulator that controls the expression of allthree operons; expression of the malT gene itself iscatabolite sensitive.a. What phenotype would you expect to result from aloss-of-function mutation in the malT gene?b. Do you expect the three maltose operons to containbinding sites for CRP (cAMP receptor protein)?Why or why not?In order to infect E. coli, bacteriophage λ binds to themaltose transport protein LamB (also known as the λreceptor protein) that is found in the outer membraneof the bacterial cell. The synthesis of LamB is induced by maltose in the medium via expression of theMalT protein, as described above.c. List the culture conditions under which wild-typeE. coli cells would be sensitive to infection bybacteriophage…A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose. What will be the effect of this mutation in the presence of allolactose (assume glucose is absent)? a) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon is blockedb) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon is blocked c) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon increasesd) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon increasesGiven the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.