In the technique of high performance liquid chromatography (HPLC) the peak area response for a particular analyte is directly proportional to concentrationAnalgesic tablets typically consist of aspirin and caffeine as the active ingredients, the remainder made up of inert binding material. The concentrations of aspirin and caffeine may beroutinely monitored using HPLC, whereby the peak areas obtained from a solution of the tablet are compared with those obtained from standard solutions of the analytes.In an analysis to determine the concentration of caffeine in such a tablet, the following data were recorded:Solutions Analysed:Tablet Sample:Mass of tablet (dissolved in 250 cm methano): 0.523 gCaffeine Standard:Mass of caffeine (diessolved in 500 cm methanol) 0.105 gHPLC results:SolutionPeak Area (catfeine)1. Tablet Sample3252. Caffeine Std.865The concentration of caffeine in the tablet (expressed as a mass (w/wl percentage) wasOa 1.89Ob 13.4O 3.77Od. 267Oe 7.54You have 3 solutions of glucose at differing concentration Solution A at 0.100 mol dm Solution Bat 0.250 mol dm sSolution Cat 0.300 mol dmt 100 cm of A was miaed with 200cm' ofland 300 cm' of C, what is the concentration of glucose in the resultant solution (in mol dm)OA 0233Ob 0250O 0200Od. 0215OR 0210Your answer is incorrectThe correct answer is0.250need help with these questions please all ofthem pl In applying the Q-test to a set of data, the value of Q (95% confidence) for 8 observations is 0.466. Glven the following two sets of dataData Set X:42.5 51.4 568 62.5 89.5 65.2 68.9 61.2Data Set Y:98.2 101.5 65.8 75.6 74.1 102.3 328 75.6Which of the data sets contain values which should be discarded (with 95% confidence)?Oa Data Set Y onlyob. Data Set X and Data Set YOC Neither Data Seto d. Data Set X onlyYou have 3 selutiom of glutose at differing concentration. Solution A at 0.100 mal dm Solution Bat 0.250 mel dm Solution C at 0300 mol dm, if 100 cm of A was mixed weh 200em'of Band 300 cm' of C. what is the concentration of gucese in the resultant solution (in mol dmOA 0233Ob. 0.2506c 0.200Od. 0215OR 0210Tuur anwer is incorrectThe correct answer i.250

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In the technique of high performance liquid chromatography (HPLC) the peak area response for a particular analyte is directly proportional to concentration Analgesic tablets typically consist of aspirin and caffeine as the active ingredients, the remainder made up of inert binding material. The concentrations of aspirin and caffeine may be routinely monitored using HPLC, whereby the peak areas obtained from a solution of the tablet are compared with those obtained from standard solutions of the analytes In an analysis to determine the concentration of caffeine in such a tablet, the following data were recorded: Solutions Analysed: Tablet Sample: Caffeine Standard: HPLC results: Mass of tablet (dissolved in 250 cm methanol: 0.523 g Mass of caffeine (dissolved in 500 cm methanol) 0.105g Solution Peak Area (caffeine) 1. Tablet Sample 325 2. Caffeine Std. 865 The conrentr ablet (expressed as a mass (ww percentage) was

In the technique of high performance liquid chromatography (HPLC) the peak area response for a particular analyte is directly proportional to concentration Analgesic tablets typically consist of aspirin and caffeine as the active ingredients, the remainder made up of inert binding material. The concentrations of aspirin and caffeine may be routinely monitored using HPLC, whereby the peak areas obtained from a solution of the tablet are compared with those obtained from standard solutions of the analytes In an analysis to determine the concentration of caffeine in such a tablet, the following data were recorded: Solutions Analysed: Tablet Sample: Caffeine Standard: HPLC results: Mass of tablet (dissolved in 250 cm methanol: 0.523 g Mass of caffeine (dissolved in 500 cm methanol) 0.105g Solution Peak Area (caffeine) 1. Tablet Sample 325 2. Caffeine Std. 865 The conrentr ablet (expressed as a mass (ww percentage) was

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter4: Least-squares And Calibration Methods

Section: Chapter Questions

Problem 3P

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Pesticide residues were found in a lot of oranges; the compound identified was the epoxide of heptachlor (EH). To quantify the amount of this pesticide in oranges, 20 grams were taken from fruit peels and mixed with methylene chloride to extract it. The concentration of heptachlor epoxide in the sample was determined by gas chromatography coupled to a detector mass (GC-MS); and calibration was performed using anthracene (A) as internal standard. For In this analysis, the anthracene had a concentration of 350 ppm and the same volume was added to each standard. The table below shows the concentrations of the standard and the ratio of the areas.and the sample is: 0.1080.Determine the concentration of heptachlor epoxide in the sample.
Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave chromatographic peak areas of 306 for X and 511 for S. A second solution containing an unknown quantity of X plus 55.5 mM S had peak areas of 251 for X and 563 for S. Find [X] in the second solution.
A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 3.40×102 and the area of peak B is determined to be 398 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.49 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 437 for peak A and 415 for peak B. What is the concentration of methyl benzoate in the plant stream?
A gas chromatogram of a mixture of toluene and ethyl (a) Measure w1/2 for each peak to the nearest 0.1 mm. When the thickness of the pen trace is significant relative to the length being measured, it is important to take the pen width into account. It is best to measure from the edge of one trace to the corresponding edge of the other trace, as shown at the bottom of the left column. (b) Find the number of theoretical plates and the plate height for each peak.
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in HPLC, what does it mean to equilibrate the system. Let us assume that the system is currently running 100%B. You have told them to run the first standard of caffeine using Reverse Phase chromatography with a C18 column, 30% A (Water/0.1%TFA) and 70% B (MeOH/0.1%TFA).What should I equilibrate the system to and for how long?
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1. What is the behavior of the resolution of the analytes as a function of the carrier gas in gas chromatography? Explain what this behavior occurs. 2. What is the behavior of the analysis time of the analytes as a function of the carrier gas in gas chromatography? Explain what this behavior occurs.. 3. Why does the order of elution of the analytes does not change when using different carrier gas in chromatography?
4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
true or false: You are using an HPLC method based on partition chromatography to separate four analytes. Each analyte has the same equilibrium constant value that describes its interaction and concentration in the stationary and mobile phases.
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