IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Based on your knowledge of the regulation of the lac operon, predict with explanation whether these gene products (B-galactosidase, lactose Permease) will be produced. a IPOZY IPOZY b. I'PO'Z'Y/IPOZY" +IPTO -IPTG B-galactosidase Permease B-galactosidase Permease Only typed answer. Will upvote you immediately. Please help

IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Based on your knowledge of the regulation of the lac operon, predict with explanation whether these gene products (B-galactosidase, lactose Permease) will be produced. a IPOZY IPOZY b. I'PO'Z'Y/IPOZY" +IPTO -IPTG B-galactosidase Permease B-galactosidase Permease Only typed answer. Will upvote you immediately. Please help

Name: IPTG can be used in the laboratory as a synthetic inducer of the lac
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Description: IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Basedon your knowledge of the regulation of the lac operon, predict with explanation whether these geneproducts (B-galactosidase, lactose Permease) will

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
The symbols A, B, C, D, E represent all the components of the lac operon, excluding LacA. The symbols are not in the order of the lac operon. Minus and plus signs represent activity of lacZ or LacY in the absence or presence of IPTG. LacI+ or LacI-, and LacO+ or LacOC alleles were used. Which of the symbols represents each of the lac operon components?
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
Suppose that you have isolated a mutant strain of E. coli inwhich the lac operon is constitutively expressed. To understandthe nature of this defect, you create a merozygote in which themutant strain contains an F′ factor with a normal lac operonand a normal lacI gene. You then compare the mutant strain andthe merozygote with regard to their β-galactosidase activities inthe presence and absence of lactose. You obtain the followingresults:
In addition to observing similarities to the lac operon, you also notice that this gene is regulated via attenuation, similar to the trp operon. Based on this similarity to this model operon, you could state that ___________. Group of answer choices If a terminator loop forms in the DNA, the expression of the structural genes is halted. The formation of the terminator hairpin followed by a series of Uracil (UUUUUUU) functions similar to Rho-Independent termination to result in the stopping of transcription. The transcription of a leader sequence affects the translation of the structural genes. Never mind – all of these statements are true! Attenuation will be the primary means of transcriptional regulation, with a repressor used as a back up option.
Give all possible genotypes of a lac operon that produces, or fails to produce, β-galactosidase and permease under the following conditions. Do not give partial-diploid genotypes. Lactose absent Lactose present β-Galactosidase Permease β-Galactosidase Permease a. − − + + b. − − − + c. − − + − d. + + + + e. − − − − f. + − + − g. − + − +
Imagine that a new strain of E. coli is discovered, and it contains the following novel genetic region including the lacI gene and the lac operon: *see image* Upstream of the promoter associated with the lac operon, there is a new operator element "Oν" (O-"nu"). This new operator has high sequence homology to O1, the DNA element that is bound with the highest affinity by the lac repressor. What would be the impact of this new genetic element regarding behavior of the operonin the absence (vs. the presence) of lactose and allolactose? Answer in terms of the protein levels of the lac repressor, as well as the proteins encoded by the genes lacZ/Y/A in the downstream region of the operon. Remember that proteins, once expressed/generated, will not persist forever, and that they have a natural lifetime (ending when they are degraded by the cell).
The figure at the beginning of this chapter showsthe binding of both a Lac repressor tetramer and aCRP-cAMP complex to the regulatory region of thelac operon.a. What is the key feature of a regulatory protein suchas the Lac repressor or CRP that allows it to regulate specifically the genes or operons it is supposedto control?b. On the figure, show the positions of the followingcomponents: (i) A Lac repressor monomer; (ii) a Lacrepressor dimer; (iii) all four DNA binding domains ofthe Lac repressor tetramer; (iv) a single helix-turnhelix motif; (v) the o1 part and either the o2 or o3 partsof the lac operator (assume the operon would be transcribed from right to left on the figure); (vi) themultimerization domains of the four Lac repressormonomers; (vii) an inducer-interacting domain; (viii)the CRP-cAMP complex; and (ix) a DNA loop.c. What is the physical basis for the formation of theDNA loop shown in the figure?d. On the figure, show the position of two axes ofsymmetry in the sequence…
Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absent
. In an effort to determine the location of an operator sitefor a negatively regulated gene, you have made a seriesof deletions within the regulatory region. The extent ofeach deletion is shown by the line underneath the sequence, and the resulting expression from the operon(i = inducible; c = constitutive; − = no expression) isalso indicated.... GGAT C T T AGCCGGCTAACATGATAAATATAA......C C T AGAATCGGCCGA TTGTA C T A TTT ATAT T ...1 i2 –3 c4 –5 ca. What can you conclude from these data about thelocation of the operator site?b. Why do you think deletions 2 and 4 show no expression of the gene?
In a bacterial cell with the genotype I-: P+: O+: Z+: Y+: A+, which of the following expression patterns of the lac operon would you see? (All + signs designate functional units and - signs designate non-functional units) A. No expression of Z, Y and A under any environmental conditions. B. Z, Y and A would always be expressed. C. Z, Y and A would be expressed when lactose was present but not expressed if lactose is not present. D. Z, Y and A would be expressed in the absence of lactose, but not when lactose was present. E. None of the above.
Suppose that a new mutation lacIes, ('es' stands for ‘extra-strength’) has been discovered in the lac operon that allows the lac-repressor to bind even to the lacOc operator. Other properties of lacIes repressor remain normal (that is the same as of lacI+). Given this genotype Is P+ Oc Z+ Y- / Ies P+ O+ Z+ Y+ , under which conditions will the gene lacZ be transcribed to produce β-galactosidase and the gene lacY transcribed to produce permease? A) With lactose, β-galactosidase and permease are produced, without lactose they are not produced B) With lactose, β-galactosidase is produced, without lactose it is not; permease is never produced C) β-galactosidase is always produced and permease is never produced D) With lactose, β-galactosidase and permease are produced, without lactose they are also produced E) β-galactosidase is always produced and permease is produced only in the presence of lactose