Isolation of DNA is a crucial step for genetic engineering. (i) What is the difference between genomic DNA and plasmid DNA? (ii) Describe the common procedure for isolating genomic DNA without using the DNA extraction kit.

Q: Based on the picture, why plasmids are used as a vector for DNA Recombination? What other vectors…

A: Ans: Recombinant DNA technology: The given process is the process of recombinant DNA technology in…

Q: What is replica plating?

A:

Q: what are advantages and disadvantages of using STRmix software in degraded DNA and DNA mixtures?

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Q: Based on the knowledge you gained from the cloning module, which of the bands in gure is associated…

A: A clone is a copy of a cell or organism that is genetically identical to the original. Clone…

Q: What is the purpose of precipitation?

A: 1. DNA is the genetic material present in most of the living organisms. The DNA is made up of 4…

Q: Can you explain what semiconservative means in relationship to the way DNA is copied?

A: In the process of DNA replication, two daughter DNAs are formed with one old and one new strand…

Q: What is the procedure to extract DNA through banana?

A: DNA (deoxyribonucleic acid) is the genetic material of the cell. It is double stranded and is made…

Q: What modifications would be recommended for easy, optimized, yield and quality DNA when plant cells…

A: Plant materials are among the most difficult materials when it comes to quality DNA extraction due…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: In order to construct a genome library, which statement most accurately describes the correct order…

A: Genomic DNA is an entity's chromosomal DNA that serves as a representation of the entirety of its…

Q: How many microliters of the pGLO plasmid do you need if you want 5 micrograms of DNA and the plasmid…

A: formula used :  DNA in ug =  (concentration of DNA / pGLO plasmid in ug/ul) ×(volume of DNA in ul)

Q: What are some important safety and ethical issues raised by this use of recombinant DNA technology?

A: Recombinant DNA (deoxyribonucleic acid) technology is the process of insertion of DNA molecules…

Q: Describe the processes involved in denaturing and renaturing of DNA, and explain what is useful…

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…

Q: How does magnetic bead DNA extraction work?

A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…

Q: How does a DNA microarray work?

A: Gene microarray technology enables to deposit many different DNA sequences on a small glass slide,…

Q: What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative…

A: DNA stands for deoxyribonucleic acid, that is a molecule that contains the directions an organism…

Q: How does gel electrophoresis separate different-sized DNA fragments?

A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…

Q: In the extraction of DNA of Banana, what do the DNA look like?

A: Introduction Bananas are elongated, edible berries produced by several species of big herbaceous…

Q: when we do plasmid DNA purification process, why we do not doing 'vortexing'?

A: DNA plasmids are small, circular, double stranded DNA molecule that are found in bacterial cells and…

Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…

A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…

Q: What are nanopores used for and tell How does DNA bind to the nanopore?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence in…

Q: What are the disadvantages of doing DNA sequencing?

A: DNA sequencing is a process of identifying the order of nucleic acid bases i.e. adenine, guanine,…

Q: Can you separate a plasmid from a non plasmid nucleic acid? How?

A: Plasmids are usually the circular, extrachromosomal DNA that has the ability of self replicating…

Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…

A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…

Q: Write the process of, building a recombinant plasmid? Or how you can build a recombinant plasmid?

A: A plasmid is a circular, single-stranded DNA molecule that is distinct from a cell's chromosomal…

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: What are dyes that stain DNA used for? Is background staining a problem when using such dyes?

A: The fragments of deoxyribonucleic acid (DNA) can be visualized by the dye after the separation…

Q: What is the importance of human dna extraction and what are the properties of DNA that can be found…

A: DNA extraction is a procedure of isolation and purification of DNA from the nucleus of cells.It was…

Q: "Plasmids are still the workhorses for many applicationsof recombinant DNA technology". Define the…

A: Introduction  Microorganisms such as bacteria, viruses, etc. are not just pathogenic and harmful but…

Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?

A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.

Q: What is used to identify the size of a DNA molecule during gel electrophoresis?

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Q: Supercoiled DNA migrates through an agorose gel faster than similarly sized linear DNA? True or…

A: According to bartleby guidelines, when multiple questions are posted. We are allowed to answer the…

Q: Calculate the purity of the plasmid DNA. Do you think the plasmid is pure? Why?

A: In molecular biology, measuring the concentration and purity is an important process for DNA…

Q: describe the two main parts of a plasmid DNA extraction and purification procedure including the…

A: Plasmid DNA extraction

Q: What is the role of a primer in DNA replication? What would happen if you forgot to add a primer in…

A: DNA replication is the process in which a parent DNA is separated and two new strands are formed…

Q: why does a higher agarose concentration render better resolution/separation of smaller DNA…

A: Electrophoresis is an essential lab procedure at the molecular level that uses an electric field to…

Q: Restriction enzymes look for palindromic sequences of DNA to cut, how does it recognize those…

A: A restriction enzyme or restriction endonuclease is an enzyme that cleaves/cuts DNA into fragments…

Q: Aside from resistance to multiple antibiotics,  what other physiologic or metabolic functions are…

A: Plasmids serve a variety of purposes. They may contain genes that help an organism survive by…

Q: What is SDS and what part does it play in the alkaline lysis method of preparing plasmid DNA from a…

A: DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural…

Q: What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic…

A: Biotechnology is innovation that uses natural frameworks, living beings or parts of this to create…

Q: What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?

A: Fluorescent labeling and radioactive labeling have found wide applications in DNA sequence analysis…

Q: If insert DNA was obtained by PCR using Taq DNA polymerase, what shape does the end of this DNA…

A: Taq polymerase is basically a thermostable polymerase that is obtained from the bacteria called as…

Q: What are plasmids? What is the importance of plasmids for the recombinant DNA technology?

A: A plasmid is circular, extrachromosomal, small piece of DNA present in the cytoplasm that is…

Q: Let’s say that you want to find out the difference in nucleotide sequence among two DNA strands, one…

A: DNA sequencing is a technique used to study the nucleotides of DNA. It is used to determine the…

Q: use a NanoDrop spectrophotometer to analyze plasmid DNA obtained from miniprep. What information…

A: A spectrophotometer is a device that measures the amount of light a solution has absorbed in order…