Will you please help me in understanding these? How many fragments will be formed after digestion in sample C, if the plasmid is circular and why? What are the critical steps in transformation?

An Illustrated Guide To Vet Med Term
4th Edition
ISBN:9781305465763
Author:ROMICH
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Chapter11: The Great Communicator
Section: Chapter Questions
Problem 22MC
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Will you please help me in understanding these?

  1. How many fragments will be formed after digestion in sample C, if the plasmid is circular and why?
  2. What are the critical steps in transformation?
  3. How was RAD52 expression observed?
  4. Provide current applications of DNA Technology
A B
kb
Eco RI
Eco RI
3.0
kb A
1.1
22
1.9
1.4
22
1.9
1.9
Eco RI
Bam HI Eco RI Bam HI
C
1.2
1.5
1.9
1.1
0.3 1.2
1.0
1.4
1.2
1.1
1.0
Bam HI
Bam HI
kb B
1.3
0.3
3.0
1.5
1.0
C.
Transcribed Image Text:A B kb Eco RI Eco RI 3.0 kb A 1.1 22 1.9 1.4 22 1.9 1.9 Eco RI Bam HI Eco RI Bam HI C 1.2 1.5 1.9 1.1 0.3 1.2 1.0 1.4 1.2 1.1 1.0 Bam HI Bam HI kb B 1.3 0.3 3.0 1.5 1.0 C.
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