Jack, a new MLT at Cape Fear Hospital was given the following information from a patient's urine sample which had been tested for the presence of S. aureus. The urine contained 6.4 x 107 CFU/ml S. aureus, the plate dilution was 10, and the sample had been taken from the 10-5 dilution tube. Calculate the number of bacterial colonies which must have been counted on the plate and what volume of the sample in µl that was actually plated to give the above results. PS, both of these values can be calculated.

Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Margaret Rodriguez, Paul Price
Chapter6: Microbial Viability And Growth
Section: Chapter Questions
Problem 4UTM
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Jack, a new MLT at Cape Fear Hospital was given the following information from a patient's urine sample which had been tested for the presence of S. aureus. The urine contained 6.4 x 107 CFU/ml S. aureus, the plate dilution was 10, and the sample had been taken from the 10-5 dilution tube. Calculate the number of bacterial colonies which must have been counted on the plate and what volume of the sample in µl that was actually plated to give the above results. PS, both of these values can be calculated.

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