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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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- If a mutation in a homeotic gene produced the following phenotypes, would you expect it to be a loss-of-function or a gain-offunction mutation? Explain your answer. A. An abdominal segment has antennae attached to it. B. The most anterior abdominal segment resembles the most posterior thoracic segment. C. The most anterior thoracic segment resembles the most posterior abdominal segment.In order to manufacture insulin for patients with diabetes, scientists create recombinant DNA by inserting a human insulin gene into bacterial DNA and have the bacteria produce human insulin. Based on what you have learned in this unit about gene expression, what must the scientists do to the DNA sequence of the human insulin gene BEFORE inserting it into the bacteria, in order to have them produce the same insulin sequence that humans would produce? Explain.Which statement about Hox genes is false? a. They are expressed in similar patterns in the embryos of both mice and flies. b. They include a conserved region of base pairs called the homeobox. c. They are arranged in similar clusters of genes in both mice and flies. d. They are part of the so-called genetic toolkit. e. Because of their important functional roles, they evolved rapidly in both mammals and flies.
- Which among the following statements is not true about mutations? * a.) It may either occur at the chromosomes or at the sequence of nucleic acids. b.) It is caused either by mutagens or through hereditary genes. c.) All mutations cause diseases and disorders in the developing organism. d.) All mutations causes a change in the structure or sequence of the genetic material.A mouse gene was identified and determined to be required for formation of heart muscle. A gene with a similar sequence was identified in the human genome. What experiment could scientists do to determine if the mouse and human genes have similar functions? A. The scientist could place the normal human gene into normal mice and see if the resulting mice are viable. B. The scientist could search the human genome for genes that encode proteins that are identical to the protein encoded by the mouse gene. C. The scientist could place the normal human gene into mutant mice to see if heart muscle forms in the mouse. D. The scientist could place the mutant mouse gene into humans to see if humans develop without heart muscle.Which of the following statements about Tbx5 is true? a. Tbx5, Tbx4, and AmphiTbx4/5 have very similar coding regions. b. Tbx5 is involved in tail development in vertebrates. c. Tbx5, Tbx4, and AmphiTbx4/5 have very similar regulatory regions. d. Tbx5 initiates hindlimb development.
- given a situation in which you manipulated a zebrafish embryo by injecting high levels of retinoic acid so that no anterior genes will be expressed. Surprisingly, after embryonic development, you are still able to observed the presence of anterior structures. What could explain this observation? What changes in your experiment would you make to achieve your objective?One unexpected result of the sequencing of the human genome was the finding that mutations in a single gene can be responsible for multiple distinct disorders. For example, mutations in the RET gene can cause two different types of multiple endocrine neoplasias, familial medullary thyroid carcinoma, and Hirschsprung disease. How do you think mutations in a single gene can have such diverse effects?At the molecular level, how do you think a gain-of-function mutation in a developmental gene might cause it to be expressed in the wrong place or at the wrong time? Explain what type of DNA sequence would be altered.
- Another way to study the role of proteins (e.g., transcription factors) that function in development is to microinject the mRNA that encodes a protein, or the purified protein itself, into an oocyte or embryo, and then determine how this affects the subsequent development of the embryo, larva, and adult. For example, if Bicoid protein is injected into the posterior region of an oocyte, the resulting embryo will develop into a larva that has anterior structures at both ends. Based on your understanding of the function of each developmental gene, what would be the predicted phenotype if the following proteins or mRNAs were injected into normal oocytes? A. Nanos mRNA injected into the anterior end of an oocyte B. Antp protein injected into the posterior end of an embryo C. Toll mRNA injected into the dorsal side of an early embryoYou found a strain of mutant fruit flies (Drosophila) living on the rotten bananas in your dorm room. You notice that many of the larvae have abnormal abdominal segments. You want to know if the “abdomenless” mutation is a maternal effect gene. Describe an experiment you would do to determine this, and the results that would support and contradict the notion that the abdomenless gene encodes a maternal determinant.In the novel Chromosome 6, by Robin Cook, a biotechnology company genetically engineers individual bonobos (a type of chimpanzee) to serve as future organ donors for clients. The genes of the bonobos are altered so that no tissue rejection takes place when their organs are transplanted into a client. What genes would need to be altered for this scenario to work? Explain your answer.