Lab 7: Protein Synthesis B. The DNA code for the A chain of insulin Below is the DNA base sequence for one part of the human insulin molecule. QUESTION: Complete the DNA molecule: add the other strand using complementary bases. TAG CAC GTC ACG TCG TGT 3' 5' 12 1 2 AGG AAC ATA 3' CCA 13 CCA 1 AGG AAC CTT 14 15 16 17 19 QUESTION: Where is DNA located within a eukaryotic cell? TAG 2 ACA ATA 6 4 5 3 CTT AAC GTT 3 TTG 4 GTT AAC 18 7 ATA ACA 8 ACA 20 C. Transcription of the Code Until the 1980s, diabetics were treated with insulin extracted from pigs or cattle. Today, genetically engineered bacteria are used to make human insulin. A human insulin gene has been added to bacteria. These bacteria are then grown in huge vats and the human insulin they produce is extracted, packed in vials, and sent to the is very similar in pharmacy for human use. This feat is possible only because the system for expressing genes all life forms. 5 60 7 CTT TTG ATA The first step in the gene expression process is transcription. When transcription is triggered by certain proteins, the DNA strand going from the 3' to 5' direction is transcribed to make mRNA. It is called the template strand. The other strand (the one you wrote out above) is not used to make mRNA but it is used when the DNA molecule is replicated. 9 QUESTION: Transcribe the gene. Write out the correct sequence of mRNA bases. Notice that this is the same gene as in B above. Recall that in RNA, thymine (T) does not exist and uracil (U) takes its place. CAC CTT GTC ACG TGT TCG TAG TTA 21 8 TAG ACA 10 ACT ACA 9 11 in m 5' TTA 3⁹ 10 ACT ACA 11 5'
Lab 7: Protein Synthesis B. The DNA code for the A chain of insulin Below is the DNA base sequence for one part of the human insulin molecule. QUESTION: Complete the DNA molecule: add the other strand using complementary bases. TAG CAC GTC ACG TCG TGT 3' 5' 12 1 2 AGG AAC ATA 3' CCA 13 CCA 1 AGG AAC CTT 14 15 16 17 19 QUESTION: Where is DNA located within a eukaryotic cell? TAG 2 ACA ATA 6 4 5 3 CTT AAC GTT 3 TTG 4 GTT AAC 18 7 ATA ACA 8 ACA 20 C. Transcription of the Code Until the 1980s, diabetics were treated with insulin extracted from pigs or cattle. Today, genetically engineered bacteria are used to make human insulin. A human insulin gene has been added to bacteria. These bacteria are then grown in huge vats and the human insulin they produce is extracted, packed in vials, and sent to the is very similar in pharmacy for human use. This feat is possible only because the system for expressing genes all life forms. 5 60 7 CTT TTG ATA The first step in the gene expression process is transcription. When transcription is triggered by certain proteins, the DNA strand going from the 3' to 5' direction is transcribed to make mRNA. It is called the template strand. The other strand (the one you wrote out above) is not used to make mRNA but it is used when the DNA molecule is replicated. 9 QUESTION: Transcribe the gene. Write out the correct sequence of mRNA bases. Notice that this is the same gene as in B above. Recall that in RNA, thymine (T) does not exist and uracil (U) takes its place. CAC CTT GTC ACG TGT TCG TAG TTA 21 8 TAG ACA 10 ACT ACA 9 11 in m 5' TTA 3⁹ 10 ACT ACA 11 5'
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter42: The Endocrine System
Section: Chapter Questions
Problem 14TYK
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Concept explainers
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Question
Transcribed Image Text:Lab 7: Protein Synthesis
B. The DNA code for the A chain of insulin
Below is the DNA base sequence for one part of the human insulin molecule.
QUESTION: Complete the DNA molecule: add the other strand using complementary bases.
TAG
CAC
ACA
ACG
TCG
12
3'
5'
AGG
12
3'
CCA
1
2
AAC ATA
13
14
3
GTT
TAG
1 2
AGG AAC ATA
CTT
4
CAC
AAC
3
GTT
13 14 15
16
15
18
QUESTION: Where is DNA located within a eukaryotic cell?
GTC
5
CTT
17
7
6
TTG ATA
C. Transcription of the Code
Until the 1980s, diabetics were treated with insulin extracted from pigs or cattle. Today, genetically engineered
bacteria are used to make human insulin. A human insulin gene has been added to bacteria. These bacteria are
then grown in huge vats and the human insulin they produce is extracted, packed in vials, and sent to the
pharmacy for human use. This feat is possible only because the system for expressing genes is very similar in
all life forms.
The first step in the gene expression process is transcription. When transcription is triggered by certain
proteins, the DNA strand going from the 3' to 5' direction is transcribed to make mRNA. It is called the
template strand. The other strand (the one you wrote out above) is not used to make mRNA but it is used when
the DNA molecule is replicated.
GTC
QUESTION: Transcribe the gene. Write out the correct sequence of mRNA bases. Notice that this is the
same gene as in B above. Recall that in RNA, thymine (T) does not exist and uracil (U) takes its place.
CCA
CTT
ACG
TAG
ACA
4 5
AAC CTT
19
ACA
60
TTG
16 17 18
TGT
7
ATA
20
19
9
8
TTA
ACA
21
TGT
8
ACA
20
TAG ACA
10
ACT
9
TCG
21
11
5'
3⁹
TTA
QUESTION: Where does transcription take place within a eukaryotic cell?
10
ACT
11
5'
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