List 3 criteria that is important to consider when choosing a recrystallization solvent In a TLC analysis, a student find that all his spots have an Rf value less than 0.05. What should the student do to achieve better separation?
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List 3 criteria that is important to consider when choosing a recrystallization solvent
In a TLC analysis, a student find that all his spots have an Rf value less than 0.05. What should the student do to achieve better separation?
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- Why is it necessary to run TLC in a closed container and to have the interior vapor saturated with the solvent? What error is introduced into the determination of an Rf value if the top is left off of the developing chamber?The identity and ratio of solvents are necessary in chromatography for achieving a good separation. What would happen if your solvent had an overly polar TLC developer? What would happen if it was overly non-polar?Explain in depth why you had to use ethanol and water to elute the compounds for column chromatography.
- 1. In thin layer chromatography (TLC), why do you need to use a pencil in marking the spots, origin, and solvent front and not a marker? 2. Why is it necessary to mark the solvent front immediately? 3. Why do you need to ensure that the loading spot is above the solvent level in paper chromatography? 4. Why do different compounds travel different distances on the piece of paper?Thin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!What is the purpose of solid-phase extraction? Why is it advantageous to use large particles (50 mm) for solid-phase extraction, but small particles (5 mm) for chromatography?
- A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the following in your drawing: Solvent front Origin Mobile phase Stationary phase Distances needed to measure Rf value for one of the spotsHelp please : When comparing HPLC with Gas Chromatography , Gas Chromatography analysis may require a chemical derivatisation step before analysis of your sample. Define chemical derivatisation, state why it is needed and briefly describe the methods that can be used to make your sample more appropriate for GC ?
- Two compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answerThin layer chromatography experiment. Show calculations for the Rf of each spot you observed.1. What factors can affect a solute's Rf value in thin-layer chromatography?2. Why is it necessary to follow these steps when conducting the experiment? -No contact with the TLC plate should be made with bare hands.-Spots should have a diameter of no more than 1 mm.-Allow sufficient solvent to saturate the chromatography chamber.