List two ways in which determining the titer of a bacterial sample is similar to that of a viral suspension. List two ways in which determining the titer of a bacterial sample is different from that of a viral suspension.
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- What is the best advise for obtaining 2-log viral inactivation when the needed CT free chlorine is 60 min mg per L, CT chloramine is 11,000 min mg per L, and CT chlorine dioxide is 170 min mg per L? Considering these virus-inactivation approaches (chlorine dioxide, chloramine, and free chlorine).when using CT values to disinfect water assets following a water quality event, what biological elements and physicochemical aspects must be considered? Display the CT curve as well as the effect of increasing pH on inactivation kinetics, and estimate the CT values that will be needed.Why is there an inverse relationship between the dilution factor and the amount of virus in solution?Calculate the viral titer of an experiment performed yielding a pfu of 279 from plating 10 ml of the 10-5 dilution. Answer in scientific notation?
- why In spread-plate technique, the bacterial suspension volume should not exceed 0.1ml.In a series of infection experiments, a researcher discovers that the ID50 value for the infectious bacterium Parasiticum mucoides is 2,000, and that the ID50 for another infectious bacterium, Donoteatum thisbacterium, is 150. Given these data, a person exposed to 1,000 bacteria of each type would be more likely to be infected by which bacterium? There is no way to know given the information provided Both infections are equally likely Donoteatum thisbacterium Parasiticum mucoidesWhat is an example of an experimental approach for determining whether a beef burger contains a food poisoning bacterium?
- What are Koch's postulates? Discuss the importance of these postulates in the identification of bacteriaElectro blotting techniques are routinely used in diagnostic microbiology for detection of a specific DNA sequence in the given bacterial culture. Explain.A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 61 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?
- Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.Name two or more situations where Koch’s postulates are impossible to complete? In other words, what factors about a microbe or factors about a disease make it impossible for Koch's postulate to be performed with that microbe or disease?An E. coli colony grew on minimal medium supplemented with arginine and leucine. However, bacteria from this colony are unable to grow and form colonies on minimal medium supplemented with arginine and methionine. What is the genotype of the bacteria in this E. coli colony?