Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series?  Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.

Q: How do you make liquid bacteria culture?

A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…

Q: How might storage of the coconut water samples at room temperature after collection and before…

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude…

A: SIM (Sulfide, Indole and Motility) test is done to differentiate the Gram negative bacilli based on…

Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…

Q: What is an aliquot? Give two uses of serial dilution.

A: Introduction: Serial dilution is a series of repeated dilution performed on the same solution to…

Q: If you perform the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?

A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…

Q: Given a log phase bacterial culture with 1 x 10^6  cells per ml and a generation time of 30 minutes…

A: Cell multiplication is due to the growth which leads to an increase in the number of bacteria over a…

Q: You perform a quadrant streak and isolate a single colony which contains two distinct species of…

A: Quadrant streaking is a technique for microbial inoculation in which a single colony is isolated on…

Q: Compare and contrast three common laboratory techniques forseparating bacteria in a mixed sample.

A: The mixture is diluted until the various individual microorganisms become separated far apart on an…

Q: What if the purpose of bacterial culture? Why might it be useful to grow bacteria?

A: According to question what is  the purpose of bacterial culture. Why might it be useful to grow…

Q: If the seven-digit code that is tabulated cannot be found in the Profile Determination Table, what…

A: The bacterial identification is important to ensure that the unknown bacterial strain is correctly…

Q: What conclusion would you draw if the numbers of bacterial colonies in were the same on the control…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: Based on the results of the Kirby-Bauer test shown below, what can we say about this strain of…

A: In Kirby-Bauer testing, bacteria are put on a plate of solid growth medium and wafers of antibiotics…

Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?

A: There are several parameters which need to be studied in microbiology about different…

Q: You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you…

A: In microscopy, oil immersion is a technique used to increase the resolving power of a microscope.…

Q: Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three…

A: E.coli cells take around 20 mins to replicate themselves. The replication of bacteria is an…

Q: 1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is…

A: Petroff- Hausser chamber is a chamber used usually for sperm and bacterial count. Number of bacteria…

Q: Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful.…

A: Quantifying viable bacteria is useful :-

Q: d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you…

A: 2d) In the initial diagram given, the number of colonies from tube 4, that were formed on the plate…

Q: A culture of bacteria is found to have a DRT of 2 minutes. How long would it take to reduce the…

A: Heat is used for sterilization of those substances that can withstand it over longer periods or…

Q: For the pour plate method, state which plate will be chosen for colony isolation and why? From the…

A: Streak plate method and pour plate method is used to isolate bacteria

Q: Attached below is the study about the effects of handwashing and the effectiveness of soap against…

A: From the figure 8 , Agar plate with bacteria; (A) depicts the results before washing hands . There…

Q: What is the name of the method you used to isolate protein from bacterial sample

A: Proteins are macromolecules shaped by amino acids. Proteins are enormous size molecules, polymers of…

Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

A: The  bacterial species are responsible for causing several diseases within the human body. So, for…

Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…

A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…

Q: Explain how to perform a serial dilution

A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…

Q: What is the importance of describing the morphological characteristics of bacterial colonies when…

A: The characteristics of colony that is formed by the bacteria are - colour, opacity, shape,…

Q: Describe how you would dilute a bacterial culture by 10-7.

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?

A: The appearance of bacteria, when stained with safranin, is to be determined.

Q: What technology is available for faster bacterial culture?

A: BASIC INFORMATION BACTERIA  It is one of the type of biological cell. They belong to prokaryotic…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: If you obtain 35 colonies after spread plating 1 ml of a 1:500 dilution of lake water, what is the…

A: A dilution can be defined as a method of addition of more solvent to a solution of a definite…

Q: If you had a liquid culture containing three different species of bacteria, how could the use of a…

A: A microbiological culture, also known as a microbial culture, is a technique for multiplying…

Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…

A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…

Q: 1. You were given a mixed nutrient agar broth culture of bacteria.  a. How will you determine the…

A: Nutrient agar medium is used to cultivate microbes supporting the growth of a wide range of…

Q: What are the advantages and limitations of studying bacteria by means of the Culture method?

A: Introduction Culture:- It is a method of growing microorganisms, tissue cells, or other living…

Q: You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which…

A: Voges-Proskauer (VP) is a test used to determine the acetoin in broth culture of bacteria. VP broth…

Q: Turbidometry is a method used for counting microbes?

A: Microbiology is the branch of biology that deals with microorganisms. Bacterial cultures and their…

Q: A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts.…

A: When the culture medium is dispersed on the viable plate, a very thin layer is formed in which…

Q: Explain why an isolated colony and a pure culture are not the samething.

A: The isolation techniques: It consists of separation of bacterial cells from other cells from mixed…

Q: Why does a streaking method used to inoculate plates result in isolated colonies? (Explain…

A:

Q: A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (in…

A: The purpose of dilution of the bacterial culture before plating is to ensure that the cells are…

Q: Explain, in your own words, why it is important to perform serial dilution when trying to quantify…

A: Serial dilution is type of dilution used in field of microbiology to identify the cell count.

Q: In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25…

A: Serial dilution of the samples is used to calculate the number of microorganisms in a sample.…

Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?

A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…