Make a comparative table, highlighting similarities and differences, between the materials: a. polyamides and b. silica gel; used as stationary phases in the technique of column chromatography. Detail the main characteristics and the most used classes for each material.
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Make a comparative table, highlighting similarities and differences, between the materials: a.
polyamides and b. silica gel; used as stationary phases in the technique of
column chromatography. Detail the main characteristics and the most used classes
for each material.
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Solved in 2 steps
- Which of the following scaffolds would make a basis for a compound library for screening? Why?Explain what each peak of the IR spectrum of the recrystallised ferrocene sample below corresponds to in great detail. Is the sample pure? Give reasons why.Since there are some inherent issues with polarimetric measurements of enantiomeric purity, suggest another technique that could be used to confirm the percent enantiomer excess.
- Outline a method from Chronology, recrystalation and liquid to liquid extraction for how you would separate a solution of the three molecules above in ethyl acetate. Explain your rationale with finding the pKa level1. Discuss the importance and precautions in using glass cuvettes. 2. Explain in your own understanding the principles of UV-Vis Spectrometer.Mass of maleic anhydride used: 5.1296 gram Volume of furan used: 3.25 ml Volume of THF used: approx. 16ml The reaction mixture was allowed to stand undisturbed for one week, whereupon, gem-like, colorless to white, prismatic crystals were isolated from the solvent matrix and allowed to air dry. When dry, the mass of the crystals was determined to be: 4.6732 gram. One small crystal was selected and ground to a fine powder for melting point measurement. The powder was observed to melt at 117 – 118 deg C. About 1ml of a saturated solution of product dissolved in DMSO-d6 was prepared and placed in an NMR tube. Subsequently, proton, carbon-13 and DEPT NMR spectra were acquired. Analysis Write the reaction using structural formulas. Calculate percent yield (show work) What does the measured melting point suggest?
- What is the structure and IUPAC name of atrazine? The herbicide simazine is more polar than atrazine. In reverse phase chromatography of a solution containing a mixture of the two substances, which would elute first? Explain why this is in terms of partition. The instrument used in this practical exercise has a UV spectrophotometer as a detector. Give two other types of chromatographic detectors that might be used.The UV–visible spectrum of GLB-1 is given in Figure 2. i.What colour(s) will the deoxy and oxy forms of GLB-1 be? Note, an electromagnetic spectrum is reproduced in the Assessment area on the S315 website. ii.By comparing the bands on the right-hand side (around 550 nm) with those of oxy- and deoxymyoglobin, suggest what type of transition gives rise to these bands.Describe in words and illustrate by means of complete chemical equations, how a mixture of 1.25 g of benzoic acid tert-butylbenzene can be separated. Apply what you learned in organic 1, reviewing in your manual the experiment of separation of 2 components by liquid-liquid extraction.
- In UV spectroscopy we are taught the effects of polarity on liquid chemistries that may undergo bathochromic red shif, hypochromic blue shift , hypsochromic decrease in absorbance and aborptivity and hyperchromic effects a increase in absorbance or absorptivity. The cause for hyperchromic shifts has one explanation that appears to make sence and that is related to Auxochromic groups or the effect on the chromophore for for a new chromophore with the auxo group that causes both a hyperchromic and bathochromic red shift. Question 1: Does this dual shift in absorbance and wavelength occur in non-liquid sustances like plastics e.g. polypropylene, polyethylene, condensation polymers like PET and Polycarbonate? Question 2: Is there a way to predict level of hyperchromic shift based on the auxochromic group or do we need to know the chromophore? Question 3: Are there known examples of chromophore and Auxochromic groups that provide various molar extinction or absorptivity or absorbance…Compound A has a lower affinity for the stationary phase thancompound B and can dissolve well in the mobile phase. In theseparation of the mixture of A and B by column chromatography,which compound will be eluted first from the column? ExplainThis is a ATR spectrum. In which caffeine/PVA films were measured. Line a) correspond to a pure pva film and the other lines are films at increasing caffeine concentration. Notice that the OH band at around 3200cm-1 decreases at higher caffeine concentrations. However at a certain concentration (line d) such band starts to increase back to the initial intensity. What does that mean? Waht is the chemical meaning for that behavior?