Match the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5 mL antibiotic solution Petri dishes 1 L of water 1 L of nutrient broth medium Talcum powder Charcoal powder [Choose ] [Choose ] Gamma radiation Autoclave Dry heat oven Filtration [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] >
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- Which of the following is false?a) A high-level disinfectant cannot be used as a sterilant.b) Critical items must be sterilized before use.c) Low numbers of endospores may remain on semicritical items.d) Standard sterilization procedures do not destroy prions.e) Quaternary ammonium compounds can be used to disinfect food preparation surfaces.Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%Define disinfection. Compare/contrast it with sterilization, antisepsis and bacteriostasis. Give the modes of action of the different antiseptics/disinfectants used in the activity (5% sodium hypochlorite (bleach), 0.05% sodium hypochlorite (bleach), 70% alcohol, 40% alcohol, Iodophor (Betadine), and Mouthwash)
- Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Oven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:Which of the following would be best for sterilizing a pre-sealed case of plastic medical supplies? A. Autoclave B. Ionization Radiation C. Sonication D. Nonionizing Radiation
- What appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobFor each item on the following list, propose a reasonable method ofsterilization. You cannot use the same method more than three timesfor the whole list. The method must sterilize, not just disinfect; and themethod must not destroy the item or render it useless unless there is noother choice. After considering a workable method, think of a methodthat would not work. Note: Where an object containing something isgiven, you must sterilize everything (for example, both the jar and thepetroleum jelly [Vaseline] in it). Some examples of sterilizing methodsare autoclave, ethylene oxide gas, dry oven, and ionizing radiation.room airseruma pot of soilplastic Petri dishesheat-sensitive drugscloth dressingsleather shoes from a thrift shopa cheese sandwicha flask of nutrient agaran entire room (walls, floor,etc.) rubber glovesdisposable syringescarcasses of cows with “madcow” diseaseinside of a refrigeratorwinea jar of Vaselinefruit in plastic bagsmilkorchid seedsmetal instrumentsmail contaminated…Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:
- Give the use and advantages of the following cultures for the examination ofcolony characteristics: a. Agar plateb. Agar slantc. Broth culturesIdentify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube