Match the terms associated with the polymerase chain reaction with their correct descriptions.Refers to the fact that DNA molecules get longer the more of them there are in the reaction.A.В.Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other.DenaturationC.Incubate the reaction at the optimal temperature for the primers to base-pair with each other.Annealing.D.Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence.Extension.Add a chaotropic agent that destabilizes hydrogen bonding.E.Amplification.F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C).G.Happens after repeated cycles of the temperature change regimen.Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen.Н.

PCR is the polymerase chain reaction in which Denaturation, Annealing and Extension process takes…

Answered: Match the terms associated with tne…

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Biology

Match the terms associated with tne polymerase chain reaction with their correct descriptions.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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Concept explainers

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.

Question

Match the terms associated with the polymerase chain reaction with their correct descriptions.
Refers to the fact that DNA molecules get longer the more of them there are in the reaction.
A.
В.
Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other.
Denaturation
C.
Incubate the reaction at the optimal temperature for the primers to base-pair with each other.
Annealing.
D.
Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence.
Extension.
Add a chaotropic agent that destabilizes hydrogen bonding.
E.
Amplification.
F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C).
G.
Happens after repeated cycles of the temperature change regimen.
Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen.
Н.

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