Place the steps of sanger sequencing in order. A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B.  ddNTPs bind and stop chain extension. C.  DNA fragments are separated by size through a capillary tube. D.  DNA polymerase copies the target region of template DNA. E.  The final nucleotide of each fragment is labeled with a fluorescent tag.

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
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Place the steps of sanger sequencing in order.

A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide.


B.  ddNTPs bind and stop chain extension.

C.  DNA fragments are separated by size through a capillary tube.


D.  DNA polymerase copies the target region of template DNA.

E.  The final nucleotide of each fragment is labeled with a fluorescent tag.

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